无缺口同系物1调控PI3K/AKT通路对鼻咽癌细胞迁移与侵袭能力的影响OACSTPCD

Objective:To explore the effects of Notchless homolog 1(NLE1)on the migration and invasion ability of nasopharyngeal carcinoma(NPC)cells through PI3K/AKT pathway.Methods:HK-1 cells and C666-1 cells were transfected with sh-NC and sh-NLE1,respectively,and divided into NC(negative control)group and sh-NLE1 group.Western blotting was used to detect the protein expression levels of NLE1,PI3K,AKT,MMP2,and MMP9 in HK-1 and C666-1 cells.The migration and invasion ability of HK-1 and C666-1 cells were detected by Transwell assay and cell scratch assay.Nude mice with xenograft tumors were used to assess the growth status of tumors.Results:Compared with the NC group,the protein expression levels of NLE1,PI3K,AKT,MMP2 and MMP9 in HK-1 and C666-1 cells in the sh-NLE1 group were significantly decreased.The migration and invasion ability of HK-1 and C666-1 cells in the sh-NLE1 group were significantly weakened compared with the NC group.Compared with the NC group,the tumor volumes of HK-1 and C666-1 cells in the sh-NLE1 group were significantly reduced.Conclusion:The inhibition of NLE1 expression in NPC cells can mediate the PI3K/AKT pathway and affect the migration and invasion ability of NPC cells,thus inhibiting the growth and development of NPC.