解剖学杂志2024，Vol.47Issue(4)：328-332,5.DOI:10.3969/j.issn.1001-1633.2024.04.009
无缺口同系物1调控PI3K/AKT通路对鼻咽癌细胞迁移与侵袭能力的影响
Effects of Notchless homolog 1 through regulating PI3K/AKT pathway on migration and invasion of nasopharyngeal carcinoma cells
徐凯 1孙晋发 1尚兴国1
作者信息
- 1. 秦皇岛市第四医院头颈外科,秦皇岛 066000
- 折叠
摘要
Abstract
Objective:To explore the effects of Notchless homolog 1(NLE1)on the migration and invasion ability of nasopharyngeal carcinoma(NPC)cells through PI3K/AKT pathway.Methods:HK-1 cells and C666-1 cells were transfected with sh-NC and sh-NLE1,respectively,and divided into NC(negative control)group and sh-NLE1 group.Western blotting was used to detect the protein expression levels of NLE1,PI3K,AKT,MMP2,and MMP9 in HK-1 and C666-1 cells.The migration and invasion ability of HK-1 and C666-1 cells were detected by Transwell assay and cell scratch assay.Nude mice with xenograft tumors were used to assess the growth status of tumors.Results:Compared with the NC group,the protein expression levels of NLE1,PI3K,AKT,MMP2 and MMP9 in HK-1 and C666-1 cells in the sh-NLE1 group were significantly decreased.The migration and invasion ability of HK-1 and C666-1 cells in the sh-NLE1 group were significantly weakened compared with the NC group.Compared with the NC group,the tumor volumes of HK-1 and C666-1 cells in the sh-NLE1 group were significantly reduced.Conclusion:The inhibition of NLE1 expression in NPC cells can mediate the PI3K/AKT pathway and affect the migration and invasion ability of NPC cells,thus inhibiting the growth and development of NPC.关键词
无缺口同系物1/鼻咽癌/磷脂酰肌醇3激酶/蛋白激酶BKey words
Notchless homolog 1/nasopharyngeal carcinoma/PI3K/AKT分类
医药卫生引用本文复制引用
徐凯,孙晋发,尚兴国..无缺口同系物1调控PI3K/AKT通路对鼻咽癌细胞迁移与侵袭能力的影响[J].解剖学杂志,2024,47(4):328-332,5.
