食品工业科技2024，Vol.45Issue(20)：187-195,9.DOI:10.13386/j.issn1002-0306.2023100230
响应面法优化山楂多酚微粒制备工艺
Optimization of Preparation Process for Hawthorn Polyphenol Microparticles Based on Response Surface Methodology
摘要
Abstract
This study synthesized hawthorn polyphenol microparticles(HPM)based on the polyelectrolyte self-assembly technology.Plackett-burman combined with Box-Behnken design was used to study the factors affecting the preparation of HPM in response to the encapsulation efficiency and drug loading of hawthorn polyphenols.The Plackett-Burman design was used to screen for factors that significantly affect the outcomes,such as preparation time,sulfuric acid concentration,and the ratio of encapsulating materials,which were optimized through Box-Behnken response surface methodology.The morphology of HPM was examined using scanning electron microscopy(SEM)and transmission electron microscopy(TEM),and their gastrointestinal release kinetics were assessed using simulated gastric and intestinal fluids.The results indicated that the preparation time,sulfuric acid concentration,and the ratio of encapsulating materials significantly effect the fabrication of HPM.An encapsulation efficiency of 96.27％±1.37％was achieved with a preparation time of 3 h,a sulfuric acid concentration of 0.0254％,and a 4:1 ratio of chitosan to sodium alginate.Morphological analysis of HPM revealed irregularly shaped,well-dispersed spherical particles.The release rate of HPM in simulated intestinal fluid was higher than in gastric fluid,with the release kinetics corresponding to zero-order in gastric fluid and first-order in intestinal fluid.In summary,this study successfully produced HPM with a high encapsulation efficiency,substantial drug loading,and good dispersion.The HPM formulated could reduce the degradation of hawthorn polyphenols in the gastrointestinal tract,offering a novel approach for incorporating hawthorn polyphenols into food products.关键词
山楂多酚/自组装/壳聚糖/海藻酸钠/工艺优化/胃肠道释放/动力学Key words
hawthorn polyphenols/self-assembly/chitosan/sodium alginate/process optimization/gastrointestinal release/kinetics分类
轻工纺织引用本文复制引用
井勇慧,刁岩,李桥,康丽,任飞燕,王东微,魏力军,徐逸尔,孙贵才,王强..响应面法优化山楂多酚微粒制备工艺[J].食品工业科技,2024,45(20):187-195,9.基金项目
西华师范大学基础研究基金(20E058) （20E058）
国家自然科学基金地区基金项目(82260870,81960881) （82260870,81960881）
江西省重点研发计划"揭榜挂帅"项目(20223BBG71S01). （20223BBG71S01）
