食品工业科技2024，Vol.45Issue(20)：332-340,9.DOI:10.13386/j.issn1002-0306.2023110259
利用斑马鱼肝癌模型探究氨基葡萄糖盐酸盐对肝癌的影响
Exploring the Effect of Glucosamine Hydrochloride on Liver Cancer Using Zebrafish Liver Cancer Model
摘要
Abstract
Objective:To explore the effects of Agaricus bisporu-derived glucosamine hydrochloride(GAH)on hepatocellular carcinoma(HCC).Methods:A zebrafish liver cancer and vascular double transgenic model,Tg(flk:mCherry;krasG12V)was induced using a 60 mg/mL doxycycline hydrochloride solution.Larvae were traced and observed for the development of liver cancer from 5 d to 10 d using laser confocal microscopy.Different concentrations of GAH were added during Dox induction,larvae in Control group(Ctr),modeling group(Dox),Dox+0.1％GAH group and Dox+0.3％GAH group(Dox)were analyzed under laser confocal microscopy to observe the effect of GAH on liver cancer and tumor vascular growth.The effect of GAH on the mRNA and protein expression of HCC and vascular-related regulatory factors(prmt5,tiam1,kat5,vegfaa,vegfr2,tgf-β1)was analyzed via qPCR and ELISA assays.Finally,the correlations between tgf-β1,prmt5,tiam1,kat5,vegfaa and vegfr2 were validated through TIMER2 online bioinformatics analysis tool.Results:Continuous induction of Dox facilitated the emergence of HCC.Compared with the Dox model group,the growth and invasion of HCC were significantly inhibited after GAH treatment(P＜0.001),and the vascular disorder of HCC was greatly improved(P＜0.00l).GAH intervention markedly ameliorated vascular dysregulation associated with liver cancer(P＜0.001).qPCR and ELISA assays revealed that GAH could effectively suppress the mRNA and protein levels of genes associated with HCC and its vasculature(P＜0.05).The significance of tgf-β1 in the expression of genes related to HCC and vascular pathways was proved using the TIMER2 database.Conclusion:GAH would potentially inhibit tumor growth and invasiveness by regulating the TGF-β signaling pathway,thereby influencing the expression of genes related to HCC and inhibiting the development of HCC vasculature.关键词
氨基葡萄糖盐酸盐/斑马鱼肝癌模型/盐酸多西环素/TGF-β信号通路Key words
glucosamine hydrochlorid/zebrafish liver cancer model/doxycycline hydrochloride/TGF-β signal pathway分类
轻工纺织引用本文复制引用
吕昊坤,杨腾辉,吴启赐,潘裕添,薛钰..利用斑马鱼肝癌模型探究氨基葡萄糖盐酸盐对肝癌的影响[J].食品工业科技,2024,45(20):332-340,9.基金项目
国家自然科学基金面上项目(32370888) （32370888）
福建省自然科学基金项目(2024J09046) （2024J09046）
闽南师范大学培育项目(MSPY202101). （MSPY202101）
