食品工业科技2024，Vol.45Issue(20)：23-33,11.DOI:10.13386/j.issn1002-0306.2024030044
脂质组学解析草鱼肉在低温冷藏中的脂质变化
Lipidomics Analysis of Lipid Changes in Grass Fish during Cold Storage
摘要
Abstract
In order to identify biomarkers of lipid deterioration in grass carp(Ctenopharyngodon idella)muscle during cold storage,the lipid profile change of the grass carp muscle stored at 4 ℃ for different periods(3,6,and 9 d)was investigated by UPLC-Q Exactive HF-X.Multivariate statistical analysis and one-way ANOVA were used to screen the differential lipids during the cold storage,and then to enrich the metabolic pathways involved in the differential lipids through the KEGG database.The results showed that the pH in grass carp muscle decreased gradually,while the thiobarbituric acid reactive substance,total free fatty acid content,and volatile base total nitrogen increased gradually,reaching 0.82 pg/kg,168.22 pg/10 g and 23.63 mg/100 g,respectively,on the 9 d of cold storage.A total of 1265 lipid molecules belonging to 5 categories and 35 subclasses were identified in the chilled grass carp muscle.Glycerol phospholipids(GP)and glycerol esters(GL)were the main components of lipids in grass carp muscle.There was no significant difference in lipid profile between grass carp muscle refrigerated for 3～6 days(P＞0.05),but there were significant changes in lipid composition when refrigerated for 9 days.Fatty acyl(FA)and sphingolipids(SP)were the major different lipids in the early stage of refrigeration(3～6 days),and GP was the main lipids with significant changes in the late stage of refrigeration(6～9 days).A total of 20 different lipid molecules were screened from multivariate statistical difference analysis,and they were mainly glycerol ester and glycerol phospholipid,among which PE(16∶1e/22∶6)showed significant differences(P＜0.01).TG(4∶0/18∶0/22∶5),PC(18∶1/20∶3),and TG(18∶0/16∶0/20∶4)showed significant differences(P＜0.05),which could be used as a marker of lipid deterioration in grass carp muscle during the cold storage.Enrichment analysis of the KEGG pathway showed that sphingolipid metabolism and glycerophospholipid metabolism were involved in lipid metabolisms during the cold storage(9 d),which were essential metabolic pathways affecting the deteriorated lipid profile of chilled grass carp.In this study,the differences in lipid profiles,molecular markers of lipid degradation,and their metabolic pathways involved in lipid deterioration of grass carp muscle during cold storage were described.It would provide theoretical guidance for exploring the mechanism of lipid change and related anti-lipid degradation research.关键词
草鱼/劣变/脂质组学/差异脂质/代谢通路Key words
grass carp/deterioration/lipidomics/differential lipids/metabolism pathway分类
轻工纺织引用本文复制引用
彭斌,徐林菊,余诚玮,胡明明,钟比真,李金林..脂质组学解析草鱼肉在低温冷藏中的脂质变化[J].食品工业科技,2024,45(20):23-33,11.基金项目
国家自然科学基金项目(32060557) （32060557）
江西省自然科学基金青年基金项目(20232BAB215060) （20232BAB215060）
江西省自然科学基金杰出青年基金项目(20224ACB215010) （20224ACB215010）
赣鄱俊才支持计划-主要学科学术和技术带头人培养项目(20232BCJ22021) （20232BCJ22021）
2023年度浙江省博士后科研项目择优资助(319030) （319030）
江西省教育厅重点项目(GJJ2200310). （GJJ2200310）
