食品工业科技2024，Vol.45Issue(20)：114-123,10.DOI:10.13386/j.issn1002-0306.2023120101
硫酸化修饰对椰子吸器多糖结构和抗氧化活性的影响
Effects of Sulfated Modification on the Structural Characterization and Antioxidant Activities of Coconut(Cocos nucifera L.)Haustorium Polysaccharide
摘要
Abstract
To investigate the effect of sulfated modification on the structure and function of coconut haustorium polysaccharide(CHP),three kinds of sulfated coconut haustorium polysaccharides(SCHP-2,SCHP-3,and SCHP-4)were prepared using sulfation reagents containing different ratios of chlorosulfonic acid and pyridine(v/v,mL/mL:1/2,1/3,and 1/4)after extracting CHP from the raw materials of coconut haustorium,and their basic chemical compositions,structural characteristics and antioxidant activity in vitro were compared and analyzed.Results showed that all three sulfation reagents were able to modify CHP(characteristic peaks of S=O and C-O-S were observed near 1100～1250 cm-1 and 820 cm-1 in infrared spectra,respectively),and the degree of substitution(DS)was the highest(0.75)at the ratio of chlorosulfonic acid and pyridine for 1/2.The total sugar,uronic acid content,and molecular weight of the three SCHP decreased,and the molar content of monosaccharides also changed when comparing with that of CHP.The thermal stability had slightly weakened as well(the degradation temperature of polysaccharides decreased from 211.17 ℃ and 282.01 ℃ of CHP to 194.86～207.70℃ and 233.35～262.46℃ of SCHP,respectively).The results of antioxidant experiments in vitro showed that the scavenging activity of SCHP on DPPH and hydroxyl radicals was lower than that of CHP,and the larger the DS was,the more obvious the decrease in activity was.In terms of the ABTS+radicals scavenging ability and ferrous ions chelating activity,increasing DS could enhance the activity of SCHP.At 8 mg/mL,the ABTS+radicals scavenging rate(54.82％)and ferrous ions chelating rate(15.59％)of SCHP-2 were significantly(P＜0.05)higher than CHP(33.67％and 8.86％).These results would provide the support for the modification and functional application of coconut haustorium polysaccharides.关键词
椰子吸器多糖/硫酸化/取代度/抗氧化活性/结构特性Key words
coconut haustorium polysaccharide/sulfation/substitution degree/antioxidant activity/structure property分类
轻工纺织引用本文复制引用
阚金涛,皮正林,杨锴莉,赵津好,刘笑焱,张玉锋..硫酸化修饰对椰子吸器多糖结构和抗氧化活性的影响[J].食品工业科技,2024,45(20):114-123,10.基金项目
海南省自然科学基金(322MS141) （322MS141）
中央级公益性科研院所基本科研业务费专项(1630152022002,1630142022009) （1630152022002,1630142022009）
海南省重点研发计划(ZDYF2021XDNY150) （ZDYF2021XDNY150）
中国热带农业科学院国家热带农业科学中心科技创新团队(CATASCXTD202304). （CATASCXTD202304）
