基于指纹图谱和量值传递关系评价姜石颗粒关键生产工艺OACSTPCD
Evaluation on Key Production Process of Jiangshi Granules Based on Fingerprint and Quantity-Value Transfer Relationship
目的 基于指纹图谱和量值传递关系相结合模式,对姜石颗粒的关键生产工艺进行评价.方法 采用HPLC建立姜石颗粒提取液、浸膏、颗粒的指纹图谱,以指纹图谱共有峰传递数、多成分含量转移率为指标,分析生产环节中量值传递规律,评价姜石颗粒制备工艺设计的合理性.结果 建立了10批姜石颗粒提取液、浸膏、颗粒的指纹图谱,共标定17个共有峰并指认出8个峰,分别为党参苷Ⅰ、甘草苷、党参炔苷、芹糖异甘草苷、异甘草苷、甘草素、甘草酸、白术内酯Ⅲ;8个成分的饮片-提取液平均转移率分别为29.42%、51.26%、23.81%、34.45%、28.29%、30.22%、42.67%、26.10%,提取液-浸膏平均转移率分别为50.05%、60.04%、51.04%、50.27%、47.60%、52.44%、53.44%、44.97%,浸膏-颗粒平均转移率分别为64.83%、78.74%、70.16%、66.56%、70.62%、69.59%、76.97%、66.43%.结论 本研究建立的姜石颗粒提取液、浸膏、颗粒指纹图谱稳定可靠,强调中药制剂研究过程的整体性,可为姜石颗粒在生产过程中的质量控制提供依据.
Objective To evaluate the key production process of Jiangshi Granules based on the new mode of combining fingerprint and quantity-value transfer relationship.Methods The fingerprints of Jiangshi Granules extract,extractum and granules were established by HPLC.The common peak transfer number of the fingerprint,the calculation of multi-component content transfer rate,and the paste rate were set as indicators to analyze the quantity and mass transfer law in the production process.The rationality of the preparation process design of Jiangshi Granules was evaluated.Results The fingerprints of 10 batches of Jiangshi Granules extract,extractum and granules were established.17 common peaks were calibrated and 8 peaks were identified.They were respectively tangshenoside I,liquiritin,lobetyolin,isoliquiritin apioside,isoliquiritin,liquiritigenin,glycyrrhizic acid,atractylenolide Ⅲ;the average transfer rates of 8 components from decoction pieces to extract were 29.42%,51.26%,23.81%,34.45%,28.29%,30.22%,42.67%,26.10%;the average transfer rates of extract to extractum were 50.05%,60.04%,51.04%,50.27%,47.60%,52.44%,53.44%,44.97%;the average transfer rates of extractum to granules were 64.83%,78.74%,70.16%,66.56%,70.62%,69.59%,76.97%,66.43%.Conclusion The established fingerprint of Jiangshi Granules extract,extractum and granules is stable and reliable,which emphasizes the integrity of the research process of TCM preparations and provides a basis for the quality control of Jiangshi Granules in the production process.
张红伟;党文飞;周晶晶;周洁;靳子明;窦霞
甘肃中医药大学药学院,甘肃 兰州 730000甘肃中医药大学附属医院,甘肃 兰州 730020
中医学
姜石颗粒指纹图谱生产工艺含量测定转移率量值传递
Jiangshi Granulesfingerprintproduction processcontent determinationtransport ratevalue dissemination
《中国中医药信息杂志》 2024 (010)
135-141 / 7
国家自然科学基金(82360944);甘肃省科技厅科技重大专项-2022年中医药产业创新联合体项目(22ZD6FA021-4);兰州市城关区科学技术局课题(2021-2-9);甘肃省中医药科研重点课题(GZKP-2022-23);甘肃中医药大学附属医院院内创新基金项目(gzfy-2020-15)
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