河南农业大学学报2024，Vol.58Issue(5)：736-745,10.DOI:10.16445/j.cnki.1000-2340.20240826.002
烟草NtPXG基因克隆、表达特征及其功能分析
Cloning,expression and functional analysis of NtPXG gene in tobacco
摘要
Abstract
[Objective]The NtPXG gene was cloned in order to investigate the response of the tobacco PXG gene to biotic and abiotic stresses,as well as to plant growth regulators.This will provide a reference for the mechanism of action of the NtPXG gene in response to tobacco mosaic virus(TMV).[Method]The relevant proteins were screened using the protein structural domain(pfarm:PLN02648),and the NtPXG gene with high expression was selected and cloned to obtain its coding region sequence,which was analysed and predicted using bioinformatics software.The expression pattern of the NtPXG gene was analysed by qRT-PCR at different developmental stages,in different organs,and under mechanical damage,drought,and different plant growth regulators treatments.The gene function was verified by overexpressing the NtPXG gene in tobacco by leaf disc transformation technology.[Result]The NtPXG gene was cloned by homologous cloning method.The gene structure and protein properties were analyzed by bioinformatics method.The expression patterns of NtPXG gene in tobacco were analyzed by qRT-PCR under different growth stage tissues,mechanical injury,drought and plant growth regulators.NtPXG gene overexpressed tobacco was obtained by leaf disc transformation and gene function analysis was performed.[Conclusion]NtPXG plays a role in the growth and development of tobacco,as well as in the response of tobacco to biotic and abiotic stresses.It is postulated that NtPXG may respond to external stresses through plant growth regulator signaling.Furthermore,overexpression of the NtPXG gene in tobacco demonstrated its inhibitory effect on TMV proliferation through JA signalling.关键词
烟草/氧脂素/生物和非生物胁迫/NtPXG基因/植物生长调节剂信号Key words
tobacco/oxytocin/biotic and abiotic stress/NtPXG gene/hormone signaling分类
生物科学引用本文复制引用
金维环,王权,王昊阳,刘磊,丁超,王明鑫,李俊营,郭红祥..烟草NtPXG基因克隆、表达特征及其功能分析[J].河南农业大学学报,2024,58(5):736-745,10.基金项目
国家自然科学基金项目(3170090209) （3170090209）
平顶山市烟草公司科技项目(PYKJ202206) （PYKJ202206）
