食品与发酵工业2024，Vol.50Issue(20)：27-34,41,9.DOI:10.13995/j.cnki.11-1802/ts.038005
利用结合态亚油酸转化生成共轭亚油酸的乳酸菌筛选
Screening for lactic acid bacteria that convert conjugated linoleic acid from non-free linoleic acid
摘要
Abstract
Conjugated linoleic acid(CLA)is a geometric and positional isomer of linoleic acid with diverse physiological functions.Previous research mainly used free linoleic acid as a substrate to produce CLA.To access the abundant non-free linoleic acid in nature,this study investigated 22 strains of lactic acid bacteria to identify those capable of producing CLA from non-free linoleic acid.Results showed that 8 strains of lactic acid bacteria efficiently produced CLA from non-free linoleic acid,with Bifidobacterium breve CCFM683 ex-hibiting the highest conversion rate at 96％.To elucidate this phenomenon,two candidate genes,Lipase A and B,were selected from the genome of Bifidobacterium breve CCFM683 through sequence comparison and expressed in Escherichia coli BL21 for functional analysis.Results showed that Lipase B specifically hydrolyzed linoleic acid monoglyceride,and the strain initially hydrolyzed the monoglyceride into free linoleic acid,which was subsequently converted into CLA when used as a substrate.This study provides a valuable reference for future research on the utilization of non-free linoleic acid by lactic acid bacteria.关键词
结合态亚油酸/共轭亚油酸/乳酸菌/脂肪酶/生物转化Key words
non-free linoleic acid/conjugated linoleic acid/lactic acid bacteria/lipase/biotransformation引用本文复制引用
华凌燕,梅永超,杨波,赵建新,陈卫,陈海琴..利用结合态亚油酸转化生成共轭亚油酸的乳酸菌筛选[J].食品与发酵工业,2024,50(20):27-34,41,9.基金项目
国家自然科学基金(32072186) （32072186）
