食品与发酵工业2024，Vol.50Issue(20)：35-41,7.DOI:10.13995/j.cnki.11-1802/ts.037752
凝结芽胞杆菌(Bacillus coagulans)分类变迁与鉴定
Taxonomic changes and identification of Bacillus coagulans
摘要
Abstract
This paper studied taxonomic changes of Bacillus coagulans,previously reclassified as Weizmannia coagulans,which cur-rently correct name is Heyndrickxia coagulans.The study was investigated by a genome-based taxonomic classification that included,in ad-dition to re-evaluation of the 16S rRNA gene sequence of CICC 20138,determination of core genome multilocus sequence typing(cgMLST),average nucleotide identity(ANI),and digital DNA-DNA hybridization(dDDH)analysis to species level.The phenotypic information of CICC 20138 was obtained by morphological,physiological,and biochemical analysis.Based on the analysis for the similari-ties of 16S rRNA gene sequences,the highest similarity to Heyndrickxia coagulans and Heyndrickxia faecalis was 99.20％.The genome size of CICC 20138(GCA_009936615.1)was 3 386 433 bp,containing 216 contigs,and the genomic DNA G+C content was 46.7 mol％.The cgMLST analysis showed the closest genetic relationship between CICC 20138 and Heyndrickxia coagulans clustered on the same branch with 100％support.The ANI value of CICC 20138 and Heyndrickxia coagulans ATCC 7050T(GCA_000832905.1)was 98.16％,and the dDDH value was 87.1％.CICC 20138 could be identified as Heyndrickxia coagulans.ANI and dDDH analysis based on whole genome sequence should be an accurate and effective method for bacterial identification,thereby obtaining more accurate species classification information.For strains with potential significant value,whole genome sequence analysis is an important tool for understand-ing microbial evolution throughout classification and identification.关键词
凝结芽胞杆菌/凝结魏茨曼氏菌/凝结海恩德里克斯氏菌/分类变迁/鉴定Key words
Bacillus coagulans/Weizmannia coagulans/Heyndrickxia coagulans/taxonomic changes/identification引用本文复制引用
曹艳花,冯会粉,唐腾飞,刘蕊,辛迪,程坤,姚粟..凝结芽胞杆菌(Bacillus coagulans)分类变迁与鉴定[J].食品与发酵工业,2024,50(20):35-41,7.基金项目
国家科技资源共享服务平台-国家菌种资源库项目(NMRC-2023-4) （NMRC-2023-4）
