酱香型白酒堆积发酵过程中真菌的结构及其来源分析OA北大核心CSTPCD
Fungal community and sources of heap fermentation during Jiangxiangxing Baijiu production
堆积发酵是酱香型白酒酿造工艺中最难掌握的关键环节,该过程是环境微生物参与白酒发酵的"窗口".真菌群落被认为是酱香型白酒糖化及产酒的主要贡献者,该研究旨在揭示堆积发酵过程中真菌的演替规律及可能来源,为管理和调控堆积发酵提供生物学依据.该研究采用高通量测序技术分析真菌群落,并通过三相图和SourceTracker分析真菌来源和比例.结果表明,堆积过程中真菌多样性随时间延长显著下降,表明堆积过程对真菌的强烈选择作用.堆积过程的优势真菌主要包括:毕赤酵母属(Pichia,平均相对丰度82.75%)、酿酒酵母属(Saccharomyces,5.75%)、嗜热子囊菌(Thermoascus,2.75%)、曲霉属(Aspergillus,2.25%)和红曲霉属(Monascus,1.12%)等.溯源分析表明大曲(平均贡献比例33.14%)主要贡献霉菌等;操作环境(平均贡献比例66.71%)主要贡献酵母类微生物.该研究强调了堆积发酵对核心微生物的富集作用,大曲及操作环境共同贡献功能微生物的来源.该研究有助于更深入了解酱香型白酒堆积环境的作用.
Heap fermentation is the most challenging step in Jiangxiangxing Baijiu production and also a window for environmental mi-croorganisms to participate in fermentation.Fungal communities are considered to be the main contributors to the saccharification and fer-mentation of Jiangxiangxing Baijiu.This study aimed to examine the variability and origins of fungi in heap fermentation,offering a biologi-cal foundation for regulating and controlling heap fermentation.High-throughput sequencing technology was used to assess the fungal com-munity,and the possible sources of fungi were further analyzed by three-phase diagram comparison and SourceTracker analysis.Results showed that fungal diversity significantly decreased over time,indicating a strong selection of fungi.The dominant fungi include Pichia(average relative abundance is 82.75%),Saccharomyces(5.75%),Thermoascus(2.75%),Aspergillus(2.25%),and Monascus(1.12%).Daqu(with an average contribution ratio of 33.14%)mainly contributed molds and other microorganisms,while the operating environment(with an average contribution ratio of 66.71%)mainly provided yeast.This study emphasized the enrichment effect of heap fermentation on core microorganisms,and both Daqu and the environment contributed to the source of functional microorganisms.This study contributes to a deeper understanding of the role of the accumulation environment of Jiangxiangxing Baijiu.
张红霞;徐岩;杜海
山西师范大学生命科学学院,山西太原,030031||江南大学生物工程学院,江苏无锡,214122||山西省微生物应用技术工程研究中心,山西太原,030031江南大学生物工程学院,江苏无锡,214122
堆积发酵环境微生物微生物来源真菌群落大曲
heap fermentationenvironment microbiotamicrobial sourcefungal communityDaqu
《食品与发酵工业》 2024 (020)
42-49 / 8
国家自然科学基金项目(32201981,32172176);江苏省卓越博士后(2022ZB503)
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