市售益生菌剂产品活性检测及其模拟胃消化耐受性OA北大核心CSTPCD

One prerequisite for probiotics is to exert beneficial effects by reaching the intestinal tract with a sufficient number of viable bacteria.Therefore,the number of viable bacteria and gastrointestinal tolerance are two important indicators for evaluating probiotic prod-ucts.The main purpose of this study was to evaluate the initial activity and gastric digestion tolerance of commercial probiotic products and to explore the effects of different physiological factors on the activity of strains during gastric digestion.Firstly,the total bacteria and live bacteria content of the samples were determined by flow cytometry.Secondly,the samples were subjected to simulated digestion experi-ments under different conditions to determine the total number of live bacteria in the samples and calculate the survival rate.Results showed that the content of viable bacteria in 15 samples was different from the labelled values,but all products met international and do-mestic standards.In the simulated gastric digestion experiment,the pH value of gastric juice was the main factor affecting the activity of bacteria,while the simulated digestion speed and pepsin concentration were the secondary factors.In the same simulated gastric digestion experiment,the tolerance of different products was significantly differed.This study provides a basis for standardizing the market environ-ment and formulating relevant standards and maintains important guiding significance for consumers to choose products and producers to improve product quality.