食品与发酵工业2024，Vol.50Issue(20)：113-119,7.DOI:10.13995/j.cnki.11-1802/ts.037815
源于传统酿造郫县豆瓣Serratia marcescens GH-2代谢产红色素结构鉴定及其抑菌活性研究
Structure identification and antibacterial activity of red pigment metabolized by Serratia marcescens GH-2 from traditionally brewed Pixian Douban
摘要
Abstract
To broaden the resources of pigment-producing microorganisms,strains with the ability to produce red pigment were screened and identified from traditional brewing Pixian Douban.The red pigments were purified and characterized by silica gel column chromatography,ultraviolet visible spectrophotometry(UV-Vis),high performance liquid chromatography(HPLC),fourier transform in-frared spectrometer(FTIR),nuclear magnetic resonance(NMR),and high resolution mass spectrum(HRMS).Additionally,the anti-bacterial activity of the red pigment produced by the strain was preliminarily analyzed.Results showed that a red pigment-producing strain GH-2 was screened from Pixian Douban,and it was identified as Serratia marcescens by morphological,physiological,and biochemical characteristics and 16S rRNA gene sequence analysis.The structure of red pigment produced by S.marcescens GH-2 was identified as 2-methyl-3-pentyl prodigiosin.The prodigiosin produced by S.marcescens GH-2 has a significant inhibitory effect on Vibrio parahaemolytic-us and has a certain inhibitory effect on Staphylococcus aureus.关键词
粘质沙雷氏菌/分子鉴定/结构表征/灵菌红素/抑菌活性Key words
Serratia marcescens/molecular identification/structural characterization/prodigiosin/antibacterial activity引用本文复制引用
贺紫涵,邹玉婷,许碧涛,商艳玲,张庆..源于传统酿造郫县豆瓣Serratia marcescens GH-2代谢产红色素结构鉴定及其抑菌活性研究[J].食品与发酵工业,2024,50(20):113-119,7.基金项目
四川省自然科学基金项目(2022NSFSC0105) （2022NSFSC0105）
