食品与发酵工业2024，Vol.50Issue(20)：209-215,7.DOI:10.13995/j.cnki.11-1802/ts.037822
基于BDNF/TrkB/CREB信号通路探讨核桃油对东莨菪碱导致认知障碍大鼠的保护作用
Effects of walnut oil on scopolamine-induced cognitive impairment in rats based on BDNF/TrkB/CREB pathway
摘要
Abstract
Research has shown that nutrition and diet play a key role in improving cognitive decline and reducing the risk of dementi-a.This study aimed to investigate the effect of walnut oil on scopolamine-induced cognitive impairment in rats based on the BDNF/TrkB/CREB signaling pathway.24 SD rats were randomly divided into a control group,a model group,and a walnut oil group.First,walnut oil was gavaged for 30 days,and the other two groups were treated with equal volume of saline.On the 31 st day,the model group and walnut oil group were intraperitoneally injected with 3 mg/(kg·d)of scopolamine daily for 5 days.The learning and memory abilities were tested through new object recognition experiments and Morris water maze tests.HE staining was used to observe the pathological morphology of the hippocampus.The acetylcholine(ACh)content and acetylcholinesterase(AChE)activity in the brain tissue were detected using a re-agent kit.Western blot was used to detect synaptophysin(SYN),postsynaptic density-95(PSD-95),brain-derived neurotrophic factor(BDNF),tropomyosin-related kinase B(TrkB),and cAMP-response element binding protein(CREB)expression levels in the hippo-campus.It found that walnut oil could improve the learning and memory ability of rats in the scopolamine-induced cognitive impairment model,as well as neuronal cell arrangement,and structural integrity.It could also decrease the activity of AChE,increase the level of ACh,and significantly elevate the expression of target proteins in hippocampal tissues.This suggests that walnut oil has an effect on cogni-tive function in scopolamine-induced cognitive impairment and its possible mechanism is related to the activation of the BDNF/TrkB/CREB signaling pathway to regulate synaptic plasticity.关键词
核桃油/东莨菪碱/认知障碍/BDNF/TrkB/CREB信号通路/突触可塑性Key words
walnut oil/scopolamine/cognitive impairment/BDNF/TrkB/CREB signal pathway/synaptic plasticity引用本文复制引用
尤佳伟,田粟,孙亚凤,夏君霞,李奕阳,刘媛,杨慧,齐兵,戴胜兴,吴彦兵..基于BDNF/TrkB/CREB信号通路探讨核桃油对东莨菪碱导致认知障碍大鼠的保护作用[J].食品与发酵工业,2024,50(20):209-215,7.基金项目
河北省创新能力提升计划项目(22567667H) （22567667H）
