食品与发酵工业2024，Vol.50Issue(20)：243-251,9.DOI:10.13995/j.cnki.11-1802/ts.037742
灯笼果叶片、宿萼和果实化学成分分析及抗氧化活性评价
Chemical composition analysis and antioxidant activity evaluation of leaf,calyx,and fruit of Physalis peruviana L.
摘要
Abstract
To comprehensively evaluate and analyze the chemical composition and antioxidant activity of Physalis peruviana L.,which was the most widely cultivated plant in Physalis genus in China,the contents of total phenols and total flavonoids of extracts from leaf,calyx,and fruit of P.peruviana L.were determined using UV spectrophotometry,the compounds were identified by ultra-high per-formance liquid chromatography-mass spectrometry and the main compounds were detected.In vitro antioxidant activity was tested using DPPH free radical,ABTS cationic free radical,·OH free radical scavenging method,and iron ion reduction method.Results indicated that the total phenolic content(8.15 mg GAE/g DW)and total flavonoid content(30.43 mg RT/g DW)of the extract from the leaf of P.peruvi-ana L.were the highest,followed by the extract from the calyx,and the total content of the fruit extract was the lowest.A total of 4 fla-vonoids,3 fatty acids,1 withanolide,1 dibenzofuran,and 1 hydroxycinnamic acid were identified from the three parts.The types of com-pounds extracted from the leaf and calyx were abundant,the content of astragalin(492.03 μg/g DW)in the leaf extract was the highest,while the content of hyperoside(162.10 μg/g DW)in the calyx extract was the highest and only ferulic acid-4'-O-glucoside compounds were detected in the fruit extracts(137.37 μg/g DW).The leaf extract had good hydroxyl radical scavenging activity(SC50=0.61 mg/mL),while the calyx extract had the best ability to scavenge DPPH radicals(SC50=0.19 mg/mL),ABTS cationic radicals(0.13 mmol TE/g),and iron ion reduction(0.16 mmol Fe2+/g).The research results have enriched the secondary metabolites data of P.peruviana L.,provi-ding a scientific basis for fully utilizing the resources of P.peruviana L.and developing natural antioxidant products.关键词
灯笼果/化学成分/抗氧化/超高效液相色谱质谱联用技术Key words
Physalis peruviana L./chemical composition/antioxidant/ultra-high performance liquid chromatography-mass spectrometry引用本文复制引用
刘天悦,滕晓祎,田永涛,么亚妹,王琰博,王文蜀..灯笼果叶片、宿萼和果实化学成分分析及抗氧化活性评价[J].食品与发酵工业,2024,50(20):243-251,9.基金项目
质谱成像与代谢组学国家民委重点实验室(中央民族大学)项目(KLMSIM202205) （中央民族大学）
