食品与发酵工业2024，Vol.50Issue(20)：279-285,7.DOI:10.13995/j.cnki.11-1802/ts.038741
金纳米粒子/氧化石墨烯复合膜电化学免疫传感器的构建及其对鲜肉中马波沙星的检测
Development of an electrochemical immunosensor based on gold nanoparticle/graphene oxide composite film and its detection of marbofloxacin in fresh meat
摘要
Abstract
An electrochemical immunosensor based on glassy carbon electrode-modified gold nanoparticles/graphene oxide composite film was developed and applied to detect marbofloxacin residues in fresh meat.Gold nanoparticles/graphene oxide composite film was syn-thesized on the electrode surface by an electrodeposition method,and then the graphene oxide of composite film was reduced by an electro-chemical method.The antibody was finally immobilized.The modified process of the electrode was characterized by cyclic voltammetry.The electrochemical detection conditions of immunosensor were optimized by differential pulse voltammetry.Under the optimum conditions,marbofloxacin was quantitatively determined.The change of peak current was linearly related to the concentration of marbofloxacin in the range of 0.5-400 ng/mL with a detection limit of 0.05 ng/mL.The detection limits in pork,chicken and beef samples were 0.09,0.10,and 0.09 μg/kg respectively,and the recovery rates ranged from 82.81％to 101.99％.These results obtained are consistent with that of ultra-high performance liquid chromatography-tandem mass spectrometry.The results showed that the fabricated electrochemical immu-nosensor could be applied for the detection of marbofloxacin residues in real samples.关键词
马波沙星/金纳米粒子/氧化石墨烯/电化学免疫传感器/鲜肉Key words
marbofloxacin/gold nanoparticles/graphene oxide/electrochemical immunosensor/fresh meat引用本文复制引用
陈秀金,王雪晴,李兆周,王耀,张敏,牛华伟,刘恒言,安彪..金纳米粒子/氧化石墨烯复合膜电化学免疫传感器的构建及其对鲜肉中马波沙星的检测[J].食品与发酵工业,2024,50(20):279-285,7.基金项目
国家自然科学基金项目(31701694,31702218) （31701694,31702218）
洛阳市公益性行业科研专项(2202021A) （2202021A）
河南省科技攻关项目(242102321131,232102320298) （242102321131,232102320298）
河南省青年人才托举工程项目(2020HYTP029) （2020HYTP029）
河南省研究生教育改革与质量提升工程项目(HNYJS2020JD06) （HNYJS2020JD06）
