沈阳农业大学学报2024，Vol.55Issue(5)：523-529,7.DOI:10.3969/j.issn.1000-1700.2024.05.002
石蒜种子发育过程中形态及内含物的变化规律
Morphology and Inclusion Changes in the Development Process of Lycoris radiata Seed
摘要
Abstract
To explore the morphological and inclusion changes during the development process of Lycoris radiata and determine the appropriate harvesting period,fresh L.radiata seeds on the 7,14,21,28,35,42,49,56,63 and 70 days after flowering(DAF)were used as research materials.Based on morphological observation,the moisture content and contents(soluble sugar,soluble protein,and starch)of the seeds were determined using drying method,coomassie brilliant blue G-250 method,and anthrone colorimetric method.The results showed that:the moisture content was still high when the seeds were mature and fell off(72.47%),seed viability(83.33%),soluble sugar(61.40 mg·g-1)and starch(35.65 mg·g-1)was high,while the content of soluble protein(0.70 mg·g-1)was very low,which was consistent with the characteristics of recalcitrant seeds.Based on the characteristics of seed morphological changes,its development process can be divided into five stages:vacuolization period(7-14 DAF),liquidization period(21-28 DAF),gelatinization period(35 DAF),embryonic development period(42-56 DAF),and maturity period(63-70 DAF),with a duration of approximately 70 days.During the entire developmental period,the color of the seed coat changes from initially translucent white to brown,and finally to black;The content of starch,soluble sugar,and protein in the endosperm significantly increases with the extension of development time,while the content of moisture content decreases significantly.Among them,the 42 DAF is an important node for the development of L.radiata seeds,and the embryo structure begins to appear.The embryo matured at 56-70 DAF,the germination rate were 8.67%,14.33%and 23.33%,respectively.Combined with the changes of internal and external morphological characteristics and inclusions of L.radiata seeds during the development process,it was determined that the appropriate harvest period was 63-70 DAF,that is,it should be harvested when the seed coat turned from brown to black and the moisture content was 72.47%-74.13%,when the seeds had completed dry matter accumulation and have germination power.关键词
石蒜/顽拗性种子/种子发育/可溶性糖/淀粉/可溶性蛋白质Key words
Lycoris radiata/recalcitrant seed/seed development/soluble sugar/starch/soluble protein分类
农业科技引用本文复制引用
蔡军火,萨日娜,游欣,魏绪英,温婷,方子安,王淅..石蒜种子发育过程中形态及内含物的变化规律[J].沈阳农业大学学报,2024,55(5):523-529,7.基金项目
国家自然科学基金项目(32360420,31960327) （32360420,31960327）
江西省花卉产业技术体系专项项目(JXARS-17) （JXARS-17）
