沈阳农业大学学报2024，Vol.55Issue(5)：538-544,7.DOI:10.3969/j.issn.1000-1700.2024.05.004
新林1号杨开放式组织培养叶片再生技术研究
Techniques on Leaf-explant Regeneration Based on Open Tissue Culture of Populus cathayana×canadansis Xinlin 1
摘要
Abstract
In order to improve the efficiency and quality of poplar propagation,the Populus cathayana×canadansis Xinlin 1 were used as the object to study the types and concentrations of antimicrobial agents,inducing differentiation medium and rooting medium to establish the open tissue culture technique system.The leaves were used as the explant and sterilized with the low toxicity 75%alcohol,and the types and concentration of antimicrobial agents were screened with the chlorothalonil and the combination(chlorothalonil+sodium cefotaxime),and the bacterial inhibition system was established.The effects of 6-BA and NAA on adventitious bud induction were studied,and the effects of IBA on rooting was also studied.The results showed that the combination of chlorothalonil and cefotaxime sodium was effective as an antimicrobial agent and had less influence on the growth and differentiation of the open tissue culture of the Populus cathayana×canadansis Xinlin 1.The suitable inhibition concentration was 0.15 g·L-1 chlorothalonil+0.05 g·L-1cefotaxime sodium.The suitable differentiation medium was 0.2 mg·L-1 6-BA+0.04 mg·L-1 NAA,the induction rate was(91.57±0.21)%,and the average number of adventitious buds was 7.5±1.0.The suitable rooting medium was 1/2MS+IBA 0.3 mg·L-1.and the transplanting survival rate was over 95%.The open tissue culture technique system of the Populus cathayana×canadansis Xinlin 1 was preliminarily established,providing a technique support for the rapid propagation of open tissue culture of Populus cathayana×canadansis Xinlin 1,and also laying the foundation for the large-scale factory propagation of poplar cultivars.关键词
杨属/开放式组培/抑菌剂/不定芽分化Key words
Populus/open tissue culture/bacteriostatic agent/adventitious buds regeneration分类
农业科技引用本文复制引用
赵鑫闻,王君,彭儒胜..新林1号杨开放式组织培养叶片再生技术研究[J].沈阳农业大学学报,2024,55(5):538-544,7.基金项目
科技创新2030—重大项目(2023ZD0405603) （2023ZD0405603）
辽宁省林业和草原局依托国家林草局科技创新平台研发项目(LLC[2022]18) （LLC[2022]18）
