新林1号杨开放式组织培养叶片再生技术研究OA北大核心CSTPCD
Techniques on Leaf-explant Regeneration Based on Open Tissue Culture of Populus cathayana×canadansis Xinlin 1
为提升杨树种苗快繁效率及质量,以新林1号杨为研究对象,在开放式组培适宜抑菌剂种类及浓度筛选基础上,对不定芽诱导培养基、生根培养基等进行研究,建立新林1号杨开放式组织培养再生技术体系.以新林1号杨叶片为外植体,使用75%酒精进行外植体消毒,考察百菌清及百菌清+头孢噻肟钠组合的抑菌效果,进行抑菌剂种类及浓度的筛选,建立抑菌体系.研究在非无菌的条件下生长调节剂6-BA、NAA对新林1号杨不定芽诱导的影响,以及不同浓度IBA对不定芽生根的影响.结果表明:…查看全部>>
In order to improve the efficiency and quality of poplar propagation,the Populus cathayana×canadansis Xinlin 1 were used as the object to study the types and concentrations of antimicrobial agents,inducing differentiation medium and rooting medium to establish the open tissue culture technique system.The leaves were used as the explant and sterilized with the low toxicity 75%alcohol,and the types and concentration of antimicrobial agents were screened with t…查看全部>>
赵鑫闻;王君;彭儒胜
辽宁省杨树研究所,辽宁盖州 115200北京林业大学林木遗传育种全国重点实验室,北京 100083辽宁省杨树研究所,辽宁盖州 115200
林学
杨属开放式组培抑菌剂不定芽分化
Populusopen tissue culturebacteriostatic agentadventitious buds regeneration
《沈阳农业大学学报》 2024 (5)
538-544,7
科技创新2030—重大项目(2023ZD0405603)辽宁省林业和草原局依托国家林草局科技创新平台研发项目(LLC[2022]18)
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