医学信息2024，Vol.37Issue(19)：62-67,6.DOI:10.3969/j.issn.1006-1959.2024.19.010
青藤碱调控LncRNA-HEIH表达抑制人食管癌细胞迁移和侵袭
Sinomenine Inhibits Migration and Invasion of Human Esophageal Cancer Cells by Regulating LncRNA-HEIH Expression
摘要
Abstract
Objective To investigate the effect of sinomenine(SIN)on the migration and invasion of esophageal cancer cells and the regulation of LncRNA-HEIH.Methods Esophageal cancer TE-1 cells were treated with different concentrations of sinomenine.TE-1 cells were transfected with si-NC and si-HEIH,respectively.TE-1 cells were transfected with pcDNA and pcDNA-HEIH,respectively,and then treated with sinomenine.CCK8 was used to detect cell viability,scratch test was used to detect cell migration rate,Transwell test was used to detect the number of invasive cells,and qRT-PCR was used to detect HEIH expression.Results Sinomenine 25,50 and 100 mg/L had no significant effect on the viability of TE-1 cells(P>0.05).Under the action of sinomenine 25,50 and 100 mg/L,the migration rate and invasive cell number of TE-1 cells decreased significantly(P<0.01).Under the action of sinomenine 25,50 and 100 mg/L,the expression of HEIH in TE-1 cells decreased significantly(P<0.01).Transfection of si-HEIH significantly reduced the migration rate and the number of invasive cells of TE-1 cells(P<0.01).Overexpression of HEIH significantly reversed the effect of sinomenine on reducing the migration rate and the number of invasive cells of TE-1 cells(P<0.01).Conclusion Sinomenine inhibits the migration and invasion of esophageal cancer cells by inhibiting the expression of HEIH.关键词
青藤碱/HEIH/迁移/侵袭/食管癌Key words
Sinomenine/HEIH/Migration/Invasion/Esophageal cancer分类
医药卫生引用本文复制引用
陈中凯,伍贤权,敬家辉,龙春晖,李龙,陈伟毅..青藤碱调控LncRNA-HEIH表达抑制人食管癌细胞迁移和侵袭[J].医学信息,2024,37(19):62-67,6.基金项目
1.国家级大学生创新创业训练计划项目(编号:202212214009) （编号:202212214009）
2.2022 年度湖南省大学生创新创业训练计划项目(编号:5021) （编号:5021）
3.湖南省自然科学基金项目(编号:2022JJ50290) （编号:2022JJ50290）
4.湖南省卫生健康委科研课题(编号:202104081483) （编号:202104081483）
