|国家科技期刊平台
首页|期刊导航|中医药信息|基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品

基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品OA

Identification of Pulsatillae Radix and Its Miscible Counterfeit Based on DNA Barcoding and ITS2 Secondary Structure

中文摘要英文摘要

目的:基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品(秋牡丹、火绒草、委陵菜),从而提高白头翁材鉴定的准确性和效率.方法:提取白头翁及易混伪品植物(秋牡丹、火绒草、委陵菜)的DNA,进行PCR扩增并测序,设计特异性引物,并采用特定的分子标记,提取DNA条形码和ITS2(内转录间隔区2)区域的二级结构,使用MEGA11.0软件对白头翁及易混伪品植物进行分析研究.结果:Neighbor-Joining(NJ)树显示白头翁、秋牡丹、火绒草、委陵菜分别独立聚为一支,且白头翁、秋牡丹、火绒草、委陵菜的二级结构在整体骨架上有明显不同,因此根据ITS2碱基序列能够对白头翁及其混伪品进行鉴定.结论:利用DNA条形码及ITS2二级结构能够准确对白头翁及易混伪品(秋牡丹、火绒草、委陵菜)进行鉴定.

Objective:To identify Pulsatillae Radix and its miscible counterfeit(Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.)based on DNA barcoding and ITS2 secondary structure,aiming to improve the accuracy and efficiency of Pulsatillae Radix identification.Methods:DNA extraction was performed on Pulsatillae Radix and its miscible counterfeit plants(Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.),followed by PCR amplification and sequencing.Specific primers were designed,and molecular markers were utilized to extract DNA barcodes and ITS2(Internal Transcribed Spacer 2)region secondary structures.Analysis was conducted using MEGA 11.0 software to study and compare Pulsatillae Radix and its miscible counterfeit.Results:Neighbor-Joining(NJ)tree analysis revealed distinct clusters for Pulsatillae Radix,Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.,with visibly different ITS2 secondary structures among them.Thus,identification of Pulsatillae Radix and its miscible counterfeit can be achieved based on their respective secondary structures.Conclusion:DNA barcoding and ITS2 secondary structure analysis provide an accurate method for identifying Pulsatillae Radix and its miscible counterfeit(Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.).

张治东;郭春云;郭阿林;汤星辰;方鑫杰;王孟虎

亳州学院,安徽 亳州 236800阜阳职业技术学院,安徽 阜阳 236031河南科技学院,河南 新乡 453003

白头翁ITS2NJ树二级结构鉴定

Pulsatillae RadixITS2NJ treeSecondary structureIdentification

《中医药信息》 2024 (010)

9-13 / 5

亳州学院项目(KJ2021A1143,2021bzjyxm26)

10.19656/j.cnki.1002-2406.20241002

评论