中医药信息2024，Vol.41Issue(10)：9-13,5.DOI:10.19656/j.cnki.1002-2406.20241002
基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品
Identification of Pulsatillae Radix and Its Miscible Counterfeit Based on DNA Barcoding and ITS2 Secondary Structure
摘要
Abstract
Objective:To identify Pulsatillae Radix and its miscible counterfeit(Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.)based on DNA barcoding and ITS2 secondary structure,aiming to improve the accuracy and efficiency of Pulsatillae Radix identification.Methods:DNA extraction was performed on Pulsatillae Radix and its miscible counterfeit plants(Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.),followed by PCR amplification and sequencing.Specific primers were designed,and molecular markers were utilized to extract DNA barcodes and ITS2(Internal Transcribed Spacer 2)region secondary structures.Analysis was conducted using MEGA 11.0 software to study and compare Pulsatillae Radix and its miscible counterfeit.Results:Neighbor-Joining(NJ)tree analysis revealed distinct clusters for Pulsatillae Radix,Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.,with visibly different ITS2 secondary structures among them.Thus,identification of Pulsatillae Radix and its miscible counterfeit can be achieved based on their respective secondary structures.Conclusion:DNA barcoding and ITS2 secondary structure analysis provide an accurate method for identifying Pulsatillae Radix and its miscible counterfeit(Anemone hupehensis var.japonica,Leontopodium leontopodioides,and Potentilla chinensis Ser.).关键词
白头翁/ITS2/NJ树/二级结构/鉴定Key words
Pulsatillae Radix/ITS2/NJ tree/Secondary structure/Identification引用本文复制引用
张治东,郭春云,郭阿林,汤星辰,方鑫杰,王孟虎..基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品[J].中医药信息,2024,41(10):9-13,5.基金项目
亳州学院项目(KJ2021A1143,2021bzjyxm26) （KJ2021A1143,2021bzjyxm26）
