中国水产科学2024，Vol.31Issue(8)：940-953,14.DOI:10.12264/JFSC2024-0006
凡纳滨对虾盐碱水养殖池塘浮游细菌群落变化特征
Dynamic changes of bacterioplankton communities in Litopenaeus vannamei farming pond using saline-alkaline water
摘要
Abstract
The use of saline-alkaline water for aquaculture has become a notable method for the comprehensive utilization of water and land resources in saline-alkaline soil.Litopenaeus vannamei is an important species in saline-alkaline aquaculture.The microbial community in water plays an important role in aquaculture processes.However,the dynamic changes of bacterioplankton throughout the entire process in shrimp farming ponds in saline-alkaline soil should be investigated to reveal the microbiota characteristics.In the present study,16S rRNA high-throughput sequencing technology and Illumina MiSeq sequencing platforms were used to analyze the bacterioplankton in the water samples from saline-alkaline L.vannamei aquaculture ponds in the Hetao Plain of Inner Mongolia.Forty-three phyla and 1004 bacterial genera were detected in samples collected at seven time points.Firmicutes(33.82％),Proteobacteria(25.82％),Cyanobacteria(17.36％),and Actinobacteria(17.36％)were the phyla with more than 10％abundance and they showed significant differences between different samples.At the genus level,the dominant genera were Exiguobacterium,Psychrobacter,and Cyanobium_PCC-6307 and their abundance were significantly different among all groups.Exiguobacterium were enriched during the early and middle stage(0-30 d,19.57％-55.17％),with less relative abundance during the later stage(40-60 d,13.32％-24.84％).Psychrobacter was less abundant on Day 0,Day 10,and Day 40(0.07％-0.53％);however,its relative abundance increased notably on Day 50(49.21％).Cyanobium_PCC-6307 showed an increase in abundance on Day 40,with small fluctuations in the remaining samples(3.87％-13.12％).Planococcus had the highest relative abundance in the water before the release of shrimp(9.68％)and decreased during the later culture stage.Massilia and Chryseobacterium were specifically enriched only on Day 30.Cyanobacteria could be considered as the major threat in inland saline-alkaline water aquaculture,with a low abundance of Vibrio.Exiguobacterium and Psychrobacter can be considered as the chief probiotics in the water source and saline-alkaline pond because of their ability to inhibit the bloom of Cyanobacteria.Unique bacterioplankton communities were formed in different aquaculture stages,with water temperature and nitrogen and phosphorus nutrients driving the succession of bacterioplankton communities.关键词
内陆盐碱地水产养殖/盐碱水/凡纳滨对虾/浮游细菌/高通量测序/内蒙古河套地区Key words
inland saline-alkaline aquaculture/saline-alkaline water/Litopenaeus vannamei/bacterioplankton/high-throughput sequencing/Hetao Plain of Inner Mongolia分类
农业科技引用本文复制引用
余鑫瑞,吴桃,王浩,闫海明,韩剑钧,管卫兵..凡纳滨对虾盐碱水养殖池塘浮游细菌群落变化特征[J].中国水产科学,2024,31(8):940-953,14.基金项目
宁夏回族自治区科技重点研发计划项目(2020BBF02009) （2020BBF02009）
鄂尔多斯市科技计划项目(2022YY025). （2022YY025）
