林业科学2024，Vol.60Issue(11)：25-36,12.DOI:10.11707/j.1001-7488.LYKX20240004
松辽平原杨树人工林土壤微生物群落结构及其功能多样性的林龄差异
The Differences in Soil Microbial Community Structure and Functional Diversity among Poplar Plantations at Different Ages in the Songliao Plain
摘要
Abstract
[Objective]The aim of this paper to analyze the changes in soil microbial community structure and functional group characteristics of poplar plantations at different ages,reveal the impact of stand age on soil microorganisms,and provide a scientific basis for soil fertility maintenance and sustainable management of poplar plantations.[Method]Populus×euramevicana'I-214'plantations at ages of 2,4,7,10,and 14 years were selected in Xinmin City,Liaoning Province.The soil physical and chemical properties of the 0-20 cm soil layer were measured,and the high-throughput sequencing technology was used to analyze the differences in soil bacterial and fungal community structures and functional diversity among I-214 poplar plantations of different stand ages.[Result]The study showed that the contents of soil organic carbon,nitrate nitrogen,available potassium,and available phosphorus all exhibited a trend of decrease followed by an increase among differents ages.The dominant bacterial phyla in the soil were Proteobacteria and Acidobacteriota,and the relative abundance of Proteobacteria increased with age.The dominant fungal phyla were Ascomycota and Basidiomycota,and their relative abundances were hardly affected by stand age.The Shannon index of soil bacteria first decreased and then increased with stand age,reaching the highest at 2 years old.The redundancy analysis showed that soil bulk density,pH,available potassium and available phosphorus content were the main soil factors that significantly affected soil bacterial communities,while total nitrogen and nitrate nitrogen content were the main soil factors that significantly affected soil fungal communities.Functional prediction of soil microbial communities showed that the relative abundance of bacterial communities involved in carbon and nitrogen transformation increased with age.[Conclusion]In the I-214 poplar plantation in the Songliao Plain,the relative abundance of Proteobacteria,the dominant bacterial community in the soil,increased with the increase of age,while the relative abundance of Acidobacteria increased first and then decreased with the increase of age,but the dominant fungal community was hardly affected by stand age.Soil bulk density,pH,available potassium,available phosphorus,total nitrogen and nitrate nitrogen content are the main soil factors affecting soil microbial communities.The relative abundance of bacterial communities involved in soil nitrogen transformation functions such as soil nitrogen fixation and carbon transformation functions such as Phototrophy increased with the increase of age.The Shannon index of soil bacteria,the relative abundance of carbon transformation functional communities,and the nutrient contents of soil nitrate nitrogen,available potassium,and available phosphorus all showed a significant decreasing trend starting from the 10-year-old,indicating that the 10-year-old poplar plantation in the Songliao Plain may be at a critical change point of soil fertility,microbial community structure,and functional diversity.At this stage,it is necessary to consider adopting appropriate management measures to maintain soil fertility,microbial community structure,and functional diversity.关键词
欧美杨I-214杨/人工林/林龄/土壤微生物群落/群落结构/多样性Key words
Populus×euramericana'I-214'/plantation/stand age/soil microbial community/community structure/diversity分类
农业科技引用本文复制引用
刘相荣,孙启武,厚凌宇,庞忠义,张琰琳,丁昌俊..松辽平原杨树人工林土壤微生物群落结构及其功能多样性的林龄差异[J].林业科学,2024,60(11):25-36,12.基金项目
"十四五"国家重点研发计划课题"杨树大径材高质林分立地品种精准适配研究"(2021YFD2201205)和"欧美杨大径级工业资源材精准高效培育技术研究"(2021YFD2201201) （2021YFD2201205）
中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金项目"不同立地杨树重要性状可塑性及其形成机制研究"(CAFYBB2023QB003). （CAFYBB2023QB003）
