食品科学2024，Vol.45Issue(24)：259-265,7.DOI:10.7506/spkx1002-6630-20240508-049
辣根过氧化物酶/胆碱氧化酶玻碳电极生物传感器检测人工大豆毛油中磷脂酰胆碱含量
Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode
摘要
Abstract
A biosensor for the determination of phosphatidylcholine(PC)in artificial crude soybean oil was fabricated by immobilizing horseradish peroxidase(HRP)and choline oxidase(ChOx)onto on a glassy carbon electrode(GCE)modified with multi-walled carbon nanotube(MWCNTs),SnO2 and chitosan(CS).Under the conditions of ChOx and HRP addition each at 2.5 U and pH 7.5,the sensor showed significant electrochemical response to PC.PC concentrations in the range of 10-300 mg/L were linearly correlated with the peak current,and the detection limit was 2 mg/L.Recoveries for spiked samples ranged from 90%to 110%with relative standard deviations(RSDs)less than 4.68%.Good correlation was observed between the results of the biosensor and those of high performance liquid chromatography(HPLC)with a correlation coefficient(r)of 0.992 9.After 14 days of storage,the sensor still retained 98%of its initial current value,indicating its good stability.This study can provide theoretical support for the rapid inspection and control of vegetable oil refining.关键词
玻碳电极/生物传感器/人工大豆毛油/磷脂酰胆碱/快速检测Key words
glassy carbon electrode/biosensors/artificial crude soybean oil/phosphatidylcholine/quick detection分类
轻工业引用本文复制引用
王东华,王旭,王宇婷,高珊,王伟宁,杨福明,于殿宇..辣根过氧化物酶/胆碱氧化酶玻碳电极生物传感器检测人工大豆毛油中磷脂酰胆碱含量[J].食品科学,2024,45(24):259-265,7.基金项目
黑龙江省科研院所优秀青年项目(CZKYF2023-1-C014) （CZKYF2023-1-C014）
哈尔滨市制造业科技创新人才项目(2023CXRCCG007) （2023CXRCCG007）
