食品科学2024，Vol.45Issue(24)：108-116,9.DOI:10.7506/spkx1002-6630-20240621-147
1株多重耐药副溶血弧菌噬菌体vB_VpP_3的生物学特性及全基因组分析
Biological Characteristics and Whole Genome Analysis of vB_VpP_3,a Bacteriophage against Multi-drug Resistant Vibrio parahaemolyticus
摘要
Abstract
Objective:To isolate bacteriophages with strong lytic activity against multi-drug resistant Vibrio parahaemolyticus.Methods:Using V.parahaemolyticus as host bacteria,bacteriophages were isolated and purified from seafood,and their biological characteristics and whole genome were analyzed.Results:Phage vB_VpP_3,which was isolated from crab,had a narrow host range and high host specificity with an optimal multiplicity of infection 0.1 and a maximum adsorption rate of 93%.The one-step growth curve showed that the latent period of the phage was 15 min,the average burst size was 110 PFU,and it was highly tolerant to the environment.Phage vB_VpP_3 belonged to the order Caudovirales of the family Podoviridae with a regular icosahedral head about 60 nm in diameter and a non-contractile tail about 20 nm in length.Its genome was double-stranded linear DNA with a total length of 42 459 bp and a total GC content of 46.87%without any virulence or drug resistance genes.Conclusion:Bacteriophage vB_VpP_3 was highly safe and could be used for biological control of multi-drug resistant V.parahaemolyticus.关键词
副溶血性弧菌/噬菌体/生物防治/生物学特性/基因组学分析Key words
Vibrio parahaemolyticus/bacteriophage/biocontrol/biological characteristics/genome analysis分类
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刘青青,张明,杨雯静,刘可,李凡,李学鹏,励建荣,张德福..1株多重耐药副溶血弧菌噬菌体vB_VpP_3的生物学特性及全基因组分析[J].食品科学,2024,45(24):108-116,9.基金项目
渤海大学海洋研究院开放课题项目(BDHYYJY2024003) （BDHYYJY2024003）
