食品科学2024，Vol.45Issue(24)：128-138,11.DOI:10.7506/spkx1002-6630-20240417-166
叉分蓼多糖结构表征及其对脂多糖诱导RAW264.7细胞炎症的抑制作用
Structural Characterization of Polygonum divaricatum L.Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells
摘要
Abstract
Objective:To explore the in vitro anti-inflammatory activity and action mechanism of a polysaccharide extracted from Polygonum divaricatum L.(PSPDL).Methods:The structure of PSPDL was characterized using high-performance gel permeation chromatography(HPGPC),high-performance liquid chromatography(HPLC),Fourier transform-infrared spectroscopy(FT-IR),X-ray diffraction(XRD),and nuclear magnetic resonance spectroscopy(NMR).The in vitro anti-inflammatory activity and action mechanism of PSPDL were investigated in lipopolysaccharide(LPS)-induced RAW264.7 cells.Results:PSPDL had a relative molecular mass of 59.475 kDa and consisted mainly of mannose(Man),rhamnose(Rha),glucuronic acid(GlcA),galacturonic acid(GalA),glucose(Glc),galactose(Gal),and arabinose(Ara),with a molar ratio of 1.69:4.95:1.04:21.79:19.01:31.68:19.84.PSPDL was an α-pyran polysaccharide containing(1→4)-α-D-Glcp linkage.PSPDL inhibited the release of inflammatory factors and related gene mRNA expression,ameliorated oxidative stress,down-regulated the protein expression levels of p38,p-p38,IκB-α,p65,and p-p65.Conclusion:PSPDL exhibited anti-inflammatory activity,perhaps by regulating inflammatory mRNA expression and modulating the mitogen-activated protein kinases/nuclear factor kappa B(MAPK/NF-κB)signaling pathway.This finding provides a scientific basis for the development and utilization of PSPDL resources.关键词
叉分蓼多糖/脂多糖/RAW264.7细胞/炎症/丝裂原活化蛋白激酶/核转录因子κB信号通路Key words
Polygonum divaricatum L.polysaccharide/lipopolysaccharide/RAW264.7 cells/inflammation/mitogen-activated protein kinases/nuclear factor kappa B signaling pathway分类
轻工纺织引用本文复制引用
崔艳艳,袁永旭,郭明坤,何文兵,李明,裴世春,李大军..叉分蓼多糖结构表征及其对脂多糖诱导RAW264.7细胞炎症的抑制作用[J].食品科学,2024,45(24):128-138,11.基金项目
"十三五"国家重点研发计划"科技助力经济2020"重点专项(SQ2020YFF0407692-2020) （SQ2020YFF0407692-2020）
吉林省科技厅项目(YDZL202201ZYTS664) （YDZL202201ZYTS664）
吉林省教育厅科学研究项目(JJKH20230594) （JJKH20230594）
