食品科学2024，Vol.45Issue(24)：150-160,11.DOI:10.7506/spkx1002-6630-20240415-120
不同茯苓菌株胞外代谢产物及抗氧化活性比较
Comparison of Extracellular Metabolites and Antioxidant Activity of Different Strains of Wolfiporia hoelen(Fr.)Y.C.Dai&V.Papp
摘要
Abstract
This study examined the differences in the extracellular metabolites and in vitro antioxidant activity of 8 strains of Wolfiporia hoelen(Fr.)Y.C.Dai&V.Papp and conducted their comprehensive evaluation using principal component analysis(PCA).To investigate the major antioxidant components of W.hoelen,widely targeted metabolomics was used to analyze the composition of extracellular flavonoids and phenolic compounds.The results showed that the contents of extracellular polysaccharides,total triterpenes and sterols were the highest in strain GTR1.The contents of extracellular flavonoids and phenolics were the highest in strain GTR2.The results of in vitro antioxidant tests showed that strains F5.78,GTR2 and XJ28 had the highest capacity to scavenge 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation,1,1-diphenyl-2-picrylhydrazyl(DPPH)and hydroxyl radicals,respectively.The results of correlation analysis showed that the contents of extracellular flavonoids was highly significantly positively correlated with the DPPH radical scavenging capacity and the contents of extracellular total phenols in W.hoelen(P<0.01).Totally 55 flavonoids and 33 phenolic compounds were qualitatively detected using ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS).The relative content of each component varied among different W.hoelen strains;the most abundant flavonoid was malonyldaidzin and the most abundant phenolic component was 3-formylsalicylic acid in W.hoelen strains FLA,FGD,FGZ,FHB,GTR1,XJ28 and F5.78.The most abundant flavonoid component in strain GTR2 was kaempferol-3-O-(6-galloyl)glucoside,which was highly significantly positively correlated with the DPPH radical scavenging capacity(P<0.01).In this strain,the most abundant phenolic component was α-cyano-4-hydroxycinnamic acid,which was significantly positively correlated with the DPPH radical scavenging capacity(P<0.05).PCA showed that GTR2 scored highest among the eight strains.In conclusion,this strain has a high content of extracellular bioactive ingredients and good antioxidant activity,making it a potential candidate for the development of antioxidant products.关键词
茯苓菌株/胞外代谢产物/抗氧化活性/山柰酚-3-O-(6-没食子酰)葡萄糖苷/α-氰基-4-羟基肉桂酸/主成分分析/广泛靶向代谢组学Key words
Wolfiporia hoelen(Fr.)Y.C.Dai&V.Papp/extracellular metabolites/antioxidant activity/kaempferol-3-O-(6-galloyl)glucoside/α-cyano-4-hydroxycinnamic acid/principal component analysis/widely targeted metabolomics分类
生物科学引用本文复制引用
谭云,申爱荣,沈宝明,喻锦秀,刘丽娜,李赛男,谭著明..不同茯苓菌株胞外代谢产物及抗氧化活性比较[J].食品科学,2024,45(24):150-160,11.基金项目
"十四五"国家重点研发计划重点专项(2023YFD2200903) （2023YFD2200903）
湖南省林业生态保护修复及发展专项(湘财环资指[2022]63) （湘财环资指[2022]63）
湖南省农业种质资源保护与利用专项(湘财农指[2021]37 （）
湘财农指[2022]23 （）
湘财农指[2023]43) （）
