食品与机械2024，Vol.40Issue(10)：129-135,7.DOI:10.13652/j.spjx.1003.5788.2024.80090
武夷岩茶酸性茶多糖对2型糖尿病大鼠胆汁酸的调节作用机制
The regulatory mechanism of acidic tea polysaccharides from Wuyi rock tea on bile acids in type 2 diabetic rats
吴仲 1杨江帆2
作者信息
- 1. 福建中医药大学药学院,福建 福州 350122||福建农林大学园艺学院,福建 福州 350002
- 2. 福建农林大学园艺学院,福建 福州 350002
- 折叠
摘要
Abstract
[Objective]This study aimed to investigate the effects of an acidic tea polysaccharide extracted from Wuyi Rock tea"yellow slice"on bile acids in hyperglycaemic rats.[Methods]In vitro experiments simulating artificial gastric and intestinal fluids were conducted to evaluate the binding capacity of tea polysaccharides to bile salts.The composition of bile acids in the cecal contents was analyzed using Liquid Chromotography with Mass Spectrometry(LC-MS);The relative mRNA expression of bile acid receptors FXR and TGR5 in rat liver and pancreas of rats were measured,along with Western blot analysis of colonic tissues.[Results]The in vitro experiments demonstrated that tea polysaccharides possess a significant binding capacity for both bound and free bile salts,with stronger affinity for bound bile salts.At 2.00 mg/mL,the binding rate of the tea polysaccharides to sodium cholate were found to be 57.59%and 62.62%for sodium glycylcholate and sodium taurocholate,respectively.In the cecal contents of rats,intervention with tea polysaccharides primarily increased the level of secondary bile acids,including deoxycholic acid and lithocholic acid.Additionlly,the treatment upregulated the mRNA expression of bile acid receptor FXR and TGR5 in the liver and pancreas,with no significant change observed in the expression of GLP-1.[Conclusion]The intervention with acidic tea polysaccharides from Wuyi rock tea led to an increased ratio of primary bile acids to secondary bile acids.This suggests that the more hydrophobic secondary bile acids are less effectively absorbed in the enterohepatic circulation.关键词
武夷岩茶/黄片/酸性茶多糖/2型糖尿病/胆汁酸/作用机制Key words
Wuyi rock tea/yellow slice/acidic tea polysaccharides/type 2 diabetes mellitus/bile acids/mechanism of action引用本文复制引用
吴仲,杨江帆..武夷岩茶酸性茶多糖对2型糖尿病大鼠胆汁酸的调节作用机制[J].食品与机械,2024,40(10):129-135,7.
