食品与机械2024，Vol.40Issue(10)：195-203,9.DOI:10.13652/j.spjx.1003.5788.2023.81201
火麻仁蛋白的反胶束法提取工艺、理化性质及消化率研究
Optimization of extraction process of hemp seed protein by reverse micelle method and study on its physicochemical properties and digestibility
摘要
Abstract
[Objective]The process was optimized and the physicochemical properties of the obtained hemp seed protein,extracted by using reverse micelle method,were analyzed.[Methods]The key factors affecting the extraction rate of protein,such as Aerosol-OT(AOT)mass concentration,water addition,buffer pH,KCl solution concentration,solid-liquid ratio,extraction temperature and time of pre-extraction process,and pH,KCl concentration,extraction time and temperature of post-extraction progress,were analyzed by single factor analysis and response surface optimization.The solubility,water holding capacity,oil holding capacity and digestion characteristics of the obtained protein were investigated.[Results]The optimal process conditions for the reverse micelle pre-extraction were obtained as follows:AOT mass concentration 0.090 4 g/mL,buffer pH 6.54,water addition 0.105 8 mL/mL,KCl concentration 0.05 mol/L,solid-liquid ratio 1∶30(g/mL),extraced for 60 min at 50℃.The pre-extraction rate was 71.38%.The optimal process conditions for the reverse micelle post-extraction were obtained as follows:pH 7.5,KCl concentration 1.5 mol/L,post-extraction time 45 min,temperature 27℃.The post-extraction rate was 80.59%,and the total extraction rate was 57.53%.The white and fluffy protein powder with purity of 98.12%was obtained by dialysis and freeze-drying.Its solubility changed significantly with pH values.The water holding capacity was 0.71 g/g,the oil holding capacity was 3.57 g/g,and the digestibility was 93.83%.[Conclusion]Reverse micelle method can be used to extract hemp seed protein.The characteristics of excellent oil holding capacity and high digestibility make hemp seed protein have unique advantages in food application.关键词
火麻仁/蛋白/反胶束/理化性质Key words
hemp seed/protein/reverse micelle/physicochemical property引用本文复制引用
李云飞,宋露露,郑郭芳,徐睿琦,刘鑫源,王志远,秦楠..火麻仁蛋白的反胶束法提取工艺、理化性质及消化率研究[J].食品与机械,2024,40(10):195-203,9.基金项目
山西中医药大学科技创新团队项目(编号:2022TD2008) （编号:2022TD2008）
校培育项目(编号:2022PY-TH-56) （编号:2022PY-TH-56）
企业横向(编号:20220828) （编号:20220828）
