食品与发酵工业2024，Vol.50Issue(24)：9-17,9.DOI:10.13995/j.cnki.11-1802/ts.038592
乳酸萃取支撑液膜的构建及其在乳酸菌液态培养中的应用
Construction of lactic acid extraction supporting liquid membrane with application to liquid cultivation of lactic acid bacteria
摘要
Abstract
Utilisation of alkali to eliminate lactic acid(LA)stress during lactic acid bacteria(LAB)culturing is an important way to achieve a high-density culture of LAB,but excess alkali also causes cell death due to osmotic pressure.Hence,in situ isolation of LA dur-ing cultivation is a promising method without salt stress.In this study,the feasibility of using supported liquid membrane in situ extraction of LA to achieve a high-density culture of Lactobacillus delbrueckii ssp.bulgaricus sp1.1 was evaluated.Firstly,a supported liquid mem-brane extraction culture system was constructed using a polyvinylidene difluoride(PVDF)membrane as a solid support membrane,natural deep eutectic solvents(NADES)composed of food source components as a carrier,and NaOH as the receiving phase.Results showed that the 30％oleic acid/menthol(2:1)supported liquid membrane had the best growth promotion effect of L.bulgaricus sp1.1,and produced 4.62-fold live bacteria numbers than that without extraction.Extraction of LA was achieved by the strong hydrogen bonds between the car-boxyl groups of oleic acid and the hydroxyl or carboxyl groups of LA,while menthol was responsible for the stabilisation of NADES.SLM extraction cultures allow continuous growth of L.bulgaricus sp1.1 by maintaining a stable pH above 4.3(fully inhibited pH of 4.2)com-pared to normal cultures.However,membrane stability issues prevented the long-term growth of L.bulgaricus spl.1.The NADES solvent extraction system has the potential to be used in the development of high-density cultures of LAB.关键词
乳酸菌/高密度培养/乳酸萃取/乳酸抑制/支撑液膜Key words
lactic acid bacteria/high-density culture/lactic acid extraction/lactic acid inhibition/supported liquid membrane引用本文复制引用
宋晓宁,姜静雅,曾剑华,王松,张兰威,公丕民..乳酸萃取支撑液膜的构建及其在乳酸菌液态培养中的应用[J].食品与发酵工业,2024,50(24):9-17,9.基金项目
国家自然科学基金项目(32001664,32372289) （32001664,32372289）
中国博士后科学基金项目(2021T140632) （2021T140632）
山东省泰山产业领军人才项目(LJNY202101) （LJNY202101）
