食品与发酵工业2024，Vol.50Issue(24)：84-90,7.DOI:10.13995/j.cnki.11-1802/ts.038675
转录因子NeyR调控弗氏链霉菌新霉素的生物合成
Transcription factor NeyR enhances neomycin synthesis in Streptomyces fradiae
摘要
Abstract
Neomycin is an aminoglycoside antibiotic mainly produced by Streptomyces fradiae.It is widely used in medicine,animal husbandry and other fields.It can effectively control and treat diseases caused by bacteria,and improve the growth speed and production performance of livestock and poultry.To improve the production of neomycin,the transcriptome data of the original Streptomyces fradiae SF-1 and the mutagenic high-yield strain SF-2 were analyzed by comparative transcriptomics,and the transcription factors with significant differences in transcription levels were mined,and then the recombinant plasmids were constructed by overexpression,and then the engi-neered strains were constructed by conjugation transfer.The engineering strain with significant effect on neomycin synthesis was screened by shake flask fermentation,and the high-yield neomycin producing engineering strain SF-2/pP-NeyR was finally obtained by promoter op-timization.Then the components of fermentation medium were orthogonal optimized,and the final titer of neomycin B was 11 843 U/mL.After comprehensive optimization,the titer of neomycin B increased by 29.6％compared with the initial one.The experimental results laid a foundation for the follow-up study of metabolic mechanism,the study of its interaction with Streptomyces fradiae neomycin synthesis gene cluster,the analysis and regulation of neomycin synthesis mechanism,and provided ideas for further improving the potency of neomycin.关键词
弗氏链霉菌/新霉素/比较转录组学/转录因子/启动子优化/发酵优化Key words
Streptomyces fradiae/neomycin/comparative transcriptomics/transcription factors/promoter optimization/fermentation op-timization引用本文复制引用
程一晗,李翔飞,韩如梦,刘艳,薛正莲..转录因子NeyR调控弗氏链霉菌新霉素的生物合成[J].食品与发酵工业,2024,50(24):84-90,7.基金项目
国家自然科学基金项目(32300059) （32300059）
安徽省博士后研究人员科研活动经费资助项目(2023B692) （2023B692）
