食品与发酵工业2024，Vol.50Issue(24)：107-113,7.DOI:10.13995/j.cnki.11-1802/ts.038657
丁酸梭菌高密度发酵及产孢条件优化
Optimization of high cell density fermentation conditions and sporulation of Clostridium butyricum
摘要
Abstract
Achieving high-density fermentation and increased sporulation rates are critical challenges in the fermentation of Clostridi-um butyricum.To address this issue,the present study optimized the fermentation and sporulation conditions of C.butyricum through single factor and response surface experiments,combined with a segmented pH control strategy.Results indicated that the optimal composition of the C.butyricum triangular flask scale fermentation medium was as follows:10.34 g/L of soluble starch,30.96 g/L of yeast extract,5.12 g/L of dipotassium hydrogen phosphate,4.00 g/L of sodium acetate,0.50 g/L of cysteine hydrochloride,0.80 g/L of calcium chloride,0.60 g/L of magnesium sulfate,and 0.20 g/L of manganese sulfate.The optimal fermentation conditions included a temperature of 37 ℃,an initial pH of 7.0,and an inoculum of l％.The experiment conducted on the 5 L fermentation tank showed that the maximum biomass level was achieved when the pH was maintained at 5.5.On the other hand,the optimal pH for sporulation was found to be 6.0.Therefore,a staged pH control strategy was employed.As the pH was decreased from an initial 7.0 to 5.5,ammonia solution was continuously added to maintain the pH level at 5.5 until the microbial culture entered the stable phase.The pH was then adjusted to 6.0 and maintained until the fermentation process was completed.Following this strategy,the biomass of C.butyricum cultured in a 5 L fermentation tank at 37 ℃for 20 h,reached its maximum level of 3.3 x 109 cells/mL,which was 3.23 times higher than that before optimization,with a sporulation rate of over 90％.关键词
丁酸梭菌/高密度培养/产孢条件/发酵优化Key words
Clostridium butyricum/high-density culture/sporulation/fermentation optimization引用本文复制引用
汪乐盛,李科,方莎莎,钱巧,李恒,许正宏,许泓瑜..丁酸梭菌高密度发酵及产孢条件优化[J].食品与发酵工业,2024,50(24):107-113,7.基金项目
国家科技基础资源调查专项资助(2021FY100900) （2021FY100900）
