食品与发酵工业2024，Vol.50Issue(24)：133-138,6.DOI:10.13995/j.cnki.11-1802/ts.038164
基于斑马鱼模型和体外抗氧化评价甘南牦牛乳抗氧化效果
Antioxidant effect of Gannan yak milk based on zebrafish model and antioxidant in vitro
摘要
Abstract
As a characteristic milk resource of the Qinghai-Tibet Plateau,yak milk was less researched and transformed into function-al products,which was not conducive to the development of the local economy.To investigate the antioxidant activity of Gannan yak milk,hydroxyl radicals,superoxide anions,and DPPH radicals were selected to evaluate the antioxidant effect of yak milk in vitro,and zebrafish with 3 dpf after fertilization were exposed to yak milk with different additions(12.5,25.0,50.0 μL/mL)for 24 h.The production rate of reactive oxygen species(ROS)and the activity of superoxide dismutase in zebrafish were detected by fluorescent staining,and the an-tioxidant effect in yak milk was evaluated.Results showed that yak milk had the ability to scavenge hydroxyl free radicals,DPPH free radi-cals,and superoxide anions.12.5 µL/mL,25.0 μL/mL,and 50.0 µL/mL addition of yak milk could reduce ROS production rate and increase antioxidant oxidase activity in zebrafish,which indicated that yak milk has antioxidant activity,and the antioxidant effect in yak milk was positively correlated with its mass concentration.When the addition of yak milk was 50.0 μL/mL,the antioxidant effect on ze-brafish reached 43.25％.Results showed that Gannan yak milk had antioxidant activity and could be used as a raw material for antioxidant functional food.关键词
甘南牦牛乳/斑马鱼/体内抗氧化/体外抗氧化Key words
Gannan yak milk/zebrafish/antioxidant in vivo/antioxidant in vitro引用本文复制引用
罗丽,宋礼,张晓梅..基于斑马鱼模型和体外抗氧化评价甘南牦牛乳抗氧化效果[J].食品与发酵工业,2024,50(24):133-138,6.基金项目
甘肃省青年科技基金计划项目(22JR5RP1025) （22JR5RP1025）
企业研发机构能力建设专项(22CX8JP250) （22CX8JP250）
中央引导地方科技发展资金项目(23ZYQP0001) （23ZYQP0001）
