食品与发酵工业2024，Vol.50Issue(24)：169-175,7.DOI:10.13995/j.cnki.11-1802/ts.038426
基于全基因组测序的菌株发酵粘液乳杆菌(Limosilactobacillus fermentum)FUA033的安全性和益生特性分析
Safety and probiotic characteristics of Limosilactobacillus fermentum FUA033 based on complete genome analysis
摘要
Abstract
Limosilactobacillus fermentum FUA033,a strain capable of converting ellagic acid to urolithin A in vitro,has the potential to be developed as a next-generation probiotic.In the present study,the genome of the strain was sequenced with the PacBio Sequel and Il-lumina platform to have a comprehensive understanding of the genetic architecture of L.fermentum FUA033.The whole genome sequence was analyzed to evaluate the safety and probiotic potential of the strain.The chromosome size of this strain was 2 150 545 bp with a GC content of 51.34％and 2 215 protein-coding genes.No plasmid was identified.VFDB database and ARDB database annotation results showed that L.fermentum FUA033 contained 81 virulence genes and 9 resistance genes.Due to the absence of plasmids and transposons,it was tentatively concluded that the virulence factors and resistance genes would not transfer.19 acid tolerance genes(arcA,argH,napA,etc.),16 bile salt tolerance genes(ldhA、ykpA、pstBl,etc.),11 antioxidant genes(msrA,Trxs,ahpC,etc.),and 3 cell adhesion genes were mined from the genome sequence of L.fermentum FUA033.The results preliminarily revealed the safety and probiotic properties of L.fermentum FUA033 and laid a theoretical foundation for the development and further application of the strain.关键词
发酵粘液乳杆菌/尿石素A/基因组测序/安全性/益生特性Key words
Limosilactobacillus fermentum/urolithin A/genome sequencing/safety/probiotics引用本文复制引用
母淑婷,华梓延,赵双双,房耀维,杨光,侯晓月,宫艳艳,桂家进,刘姝..基于全基因组测序的菌株发酵粘液乳杆菌(Limosilactobacillus fermentum)FUA033的安全性和益生特性分析[J].食品与发酵工业,2024,50(24):169-175,7.基金项目
江苏省第五期"333工程"科研项目(BRA2019243) （BRA2019243）
江苏省高等学校自然科学研究重大项目(20KJA550001) （20KJA550001）
江苏省研究生科研与实践创新计划项目(KYCX2023_32) （KYCX2023_32）
大学生创新创业训练计划立项项目(SY202311641638001) （SY202311641638001）
