食品与发酵工业2024，Vol.50Issue(24)：176-181,6.DOI:10.13995/j.cnki.11-1802/ts.038616
基于Illumina MiSeq高通量测序分析电子束辐照对手撕牛肉微生物多样性的影响
Effect of electron beam irradiation on microbial community diversity of shredded beef by Illumina MiSeq sequencing
摘要
Abstract
To investigate the effect of high-energy electron beam irradiation on the total bacterial count and microbial community di-versity of shredded beef,vacuum-packed shredded beef was irradiated with electron beams at different doses(0,2,5,8 kGy).This study measured the total bacterial count during storage and analyzed the community structure using Illumina MiSeq high-throughput sequencing technology.Results demonstrated that as the irradiation dose increased,the total bacterial count decreased,consequently extending the shelf life of the product.The increase in irradiation dose reduced the richness of microbial species,and the extension of storage time also led to a decrease in richness.At the phylum level,Firmicutes and Proteobacteria were the dominant microbial communities during storage across all groups.At the genus level,increasing the irradiation dose resulted in an increased abundance of Bacillus and Acinetobacter,while the abundance of Enterobacterium decreased.The control(CK)and the 2 kGy group exhibited a significant increase in the abun-dance of Weissella during storage(P＜0.05).In the 5 kGy and 8 kGy groups,the relative abundance of Bacillus significantly increased with storage time(P＜0.05).This study provides a preliminary exploration of the changes in microbial diversity during the storage of shredded beef after irradiation and offers a theoretical foundation for the application of irradiation sterilization technology in the preservation of meat products and the prediction of shelf life.关键词
电子束辐照/高通量测序/手撕牛肉/贮藏/微生物多样性Key words
electron beam irradiation/high-throughput sequencing/shredded beef/storage/microbial community diversity引用本文复制引用
徐攀,王田田,张雁君,高鹏..基于Illumina MiSeq高通量测序分析电子束辐照对手撕牛肉微生物多样性的影响[J].食品与发酵工业,2024,50(24):176-181,6.基金项目
四川省自然科学基金项目(2023NSFSC0133) （2023NSFSC0133）
四川省省院省校科技合作项目(2024YFHZ0047) （2024YFHZ0047）
