食品与发酵工业2024，Vol.50Issue(24)：201-211,11.DOI:10.13995/j.cnki.11-1802/ts.038595
不同富营养培养基对人肠道菌群的体外培养效果比较分析
Comparative analysis of in vitro cultivation effects of different rich media on human intestinal microbiota
摘要
Abstract
The emergence of culturomics,with rich media(RM)as a crucial component,provides a significant foundation for stud-ying the mechanisms of interaction between the gut microbiota and the host.To explore the in vitro cultivation effects of different RMs on the human gut microbiota,the plate counting method and high-throughput 16S rDNA sequencing technology were employed to analyze the cultivation effects of Yeast extract-casein hydro lysate-fatty acid medium(YCFA medium),Gifu anaerobic medium(GAM medium),and a patented ZJ medium on the human gut microbiota.Plate counting results showed that the total colony count of gut microbiota exhibited a trend of initially increasing and then decreasing after cultivation for different durations(0-7 days)in various culture media.The highest colony count values for the gut microbiota after cultivation in three different media were as follows:ZJ(9.88 lg CFU/mL)＞GAM(9.79 lg CFU/mL)＞YCFA(9.68 lg CFU/mL).The high-throughput sequencing results revealed a decrease in bacterial diversity after in vitro cultivation,with each culture medium selectively enriching different strains.Among them,the ZJ medium exhibited the most significant decrease in community diversity after cultivation.The β-diversity analysis indicated that the gut microbiota's composition after cultivation in the GAM medium was closer to the composition observed in the uncultivated state.Therefore,compared to YCFA and ZJ media,GAM medium is a more optimal RM medium for the in vitro cultivation of the gut microbiota.关键词
肠道菌群/富营养培养基/菌落总数/多样性分析/厌氧培养Key words
gut microbiota/rich media/total colony count/diversity analysis/anaerobic cultivation引用本文复制引用
路婧,夏雪娟,黄骏楠,陈泫羽,周璐,董庆利..不同富营养培养基对人肠道菌群的体外培养效果比较分析[J].食品与发酵工业,2024,50(24):201-211,11.基金项目
上海市浦江人才计划(23PJ1409600) （23PJ1409600）
上海理工大学创新创业训练计划项目(XJ2023328) （XJ2023328）
