食品与发酵工业2024，Vol.50Issue(24)：321-330,10.DOI:10.13995/j.cnki.11-1802/ts.039835
超高效液相色谱-四极杆飞行时间串联质谱结合GNPS分子网络技术检测抗生素类兽药残留
Detection method of antibiotic veterinary drug residues based on ultra performance liquid chromatography-quadrupole time of flight tandem mass spectrometry combined with GNPS molecular network
摘要
Abstract
To investigate the high throughput detection method of veterinary drug residues,ultra performance liquid chromatography-quadrupole time of flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)was used to detect livestock and poultry matrix samples and 21 standard veterinary drugs.The retention time and characteristic fragment ion mass spectra of each standard veterinary drug were ob-tained.The linear equation,correlation coefficient,linear range,and limit of detection were obtained.Based on the GNPS(global natural products social molecular networking)website,the secondary mass spectrometry cleavage of veterinary drugs was further explored,and the structure of the compound was analyzed and confirmed.Results showed that the cleavage pattern of the same type of veterinary drug mole-cule was similar,and the rapid high throughput screening of veterinary drug residues in livestock and poultry meat could be realized based on the secondary mass spectrometry lysis fragments of veterinary drug molecule.According to UPLC-Q-TOF MS/MS and GNPS molecular network technology,10 drugs were detected positive in 24 matrix samples of pork and fish,and 3 positive drugs were not included in the established standard veterinary drug information database.Indicating that compared with the conventional detection methods,UPLC-Q-TOF MS/MS combined with GNPS molecular network technology can realize the identification of veterinary drug residues in livestock and poul-try meat quickly and accurately,and it can also find unknown veterinary drugs.关键词
兽药残留/质谱裂解规律/GNPS分子网络/超高效液相色谱-四极杆飞行时间串联质谱Key words
veterinary drug residues/mass spectrometry fragmentation pattern/GNN PS molecular network/ultra performance liquid chro-matography-quadrupole time of flight tandem mass spectrometry引用本文复制引用
王伟,成向荣,任丽秋,方志娟,胡文涛,张芬,张雪婧,赵紫薇,马家辉,李晓芹..超高效液相色谱-四极杆飞行时间串联质谱结合GNPS分子网络技术检测抗生素类兽药残留[J].食品与发酵工业,2024,50(24):321-330,10.基金项目
江苏省市场监督管理局科研项目(KJ21125028) （KJ21125028）
苏州市科技计划项目(SS202121) （SS202121）
苏州市科技计划项目(SS202143) （SS202143）
