中国食物与营养2024，Vol.30Issue(11)：41-47,7.
基于GPC/UPLC-MS/MS定量分析鬼伞菌属中的鬼伞菌素
Quantitative Analysis of Coprine in Coprinus Based on GPC/UPLC-MS/MS
摘要
Abstract
[Objective]The analytical methods of ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and information dependent enhanced sub ion scanning (MRM-IDA-EPI) for coprine based on the pretreatment method of gel purifica-tion chromatography (GPC) were established to provide reference basis for quantitative detection and rapid screening of coprinus food borne poisoning.[Method]The samples of coprinus or blood were extracted by ultrasonic extraction with methanol-water-acetonitrile mixed solvent,separated and purified by GPC,quantitatively detected by UPLC-MS/MS,and screened and analyzed by MRM-IDA-EPI.[Result]The yield of coprine obtained by this purification method was 128 mg/kg,and the purity was HPLC level. The recoveries of low,medium and high concentrations of coprine were 87.3％—93.7％,and the precision (RSD) was 0.9％—4.2％. The linear correlation coef-ficient (r) of the coprine standard curve was>0.9990. The limit of detection (LOD,S/N=3) and limit of quantitation (LOQ) were 2 μg/kg and 6 μg/kg,respectively. The matrix effect coefficient of the GPC pre-treatment purification method was K=1.007.[Conclusion]The established GPC purification method for anamorph species of coprine had low matrix interference,and the UPLC-MS/MS and MRM-IDA-EPI analysis methods had accurate results,good reproducibility,and high sensitivity,making them suitable for analysis and screening of multiple sample types.关键词
鬼伞菌属/鬼伞菌素/凝胶净化色谱/超高效液相色谱串联质谱/MRM-IDA-EPIKey words
coprinus/coprine/gel permeation chromatography(GPC)/ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)/MRM-IDA-EPI引用本文复制引用
瞿广胜,李显娱,戴乾,徐林,韦刚,刘钰洁,蔡贵香,赵连伟..基于GPC/UPLC-MS/MS定量分析鬼伞菌属中的鬼伞菌素[J].中国食物与营养,2024,30(11):41-47,7.基金项目
贵州省科学技术基金项目(项目编号:黔科合支撑[2021]一般023) （项目编号:黔科合支撑[2021]一般023）
安顺市科技计划项目(项目编号:安市科社[2021]16号) （项目编号:安市科社[2021]16号）
安顺市科技创新平台建设项目(项目编号:安市科平[2022]1号). （项目编号:安市科平[2022]1号）
