食品科学2025，Vol.46Issue(2)：30-37,8.DOI:10.7506/spkx1002-6630-20231023-190
凡纳滨对虾肌质钙结合蛋白纯化鉴定、理化特性及抗原表位分析
Purification,Physicochemical Properties,and Epitope Analysis of Sarcoplasmic Calcium-Binding Protein from Penaeus vannamei
摘要
Abstract
Sarcoplasmic-calcium-binding protein(SCP)was isolated,purified and identified from Penaeus vannamei.Meanwhile,its physicochemical properties(immunoactivity,thermal stability,pH stability and digestive stability),secondary structures and epitopes were studied.The results showed that the purified protein,which was obtained by successive steps of crude protein extraction,ammonium sulfate fractionation and anion exchange chromatography and exhibited a molecular mass of 21.6 kDa,was identified as SCP with a peptide coverage of 93.26%.SCP had strong immunoactivity,which decreased during heat treatment(≥65℃)and under strongly acidic and alkaline conditions.SCP had strong stability against intestinal fluid digestion,but poor stability against gastric fluid digestion.The secondary structure of SCP consisted of 26%α-helix,16.9%β-sheet,17.5%β-turn,and 39.6%random coil.Eight epitopes in SCP were predicted and identified using bioinformatics tools combined with immunological techniques.关键词
凡纳滨对虾/肌质钙结合蛋白/稳定性/抗原表位Key words
Penaeus vannamei/sarcoplasmic calcium-binding protein/stability/epitope分类
轻工业引用本文复制引用
陈伟,周军君,陈雅纯,贾英民,马爱进..凡纳滨对虾肌质钙结合蛋白纯化鉴定、理化特性及抗原表位分析[J].食品科学,2025,46(2):30-37,8.基金项目
国家自然科学基金面上项目(31972184) （31972184）
