食品科学2025，Vol.46Issue(2)：50-56,7.DOI:10.7506/spkx1002-6630-20231122-173
低分子质量岩藻聚糖硫酸酯的分离纯化、结构表征及体外抗氧化活性
Separation,Purification,Structural Characterization and in Vitro Antioxidant Activity of Low Molecular Mass Fucoidans
摘要
Abstract
In this study,two low molecular mass fucoidans(LMWFs),F-1 and F-2,derived from Undaria pinnatifida fucoidan,were prepared and purified by free radical oxidation degradation and strong anion exchange chromatography.The chemical composition and structure characteristics of LMWFs were analyzed using molecular exclusion chromatography(MEC),high performance liquid chromatography(HPLC),infrared spectroscopy(IRS)and nuclear magnetic resonance spectroscopy(NMRS),and the structure-antioxidant activity relationship was explored.The results showed that F-1 and F-2 were primarily composed of fucose and galactose,with molecular mass of 2 790 and 23 510 Da,respectively.Their sulfate contents were(27.05±0.95)%and(34.73±0.08)%,and their uronic acid contents were(15.39±0.33)%and(6.76±0.38)%,respectively.In both F-1 and F-2,the configuration of glycosidic bonds was α-type,with a certain degree of acetylation and sulfation.Both LMWFs could scavenge 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)cationic radicals and showed certain reducing power in the potassium ferricyanide reduction assay.These antioxidant properties were concentration dependent.The contents of polyuronide and galactose in the glycan chain of F-1 was relatively high,so its antioxidant activity was superior to that of F-2 under the same dosage conditions.These findings provide a theoretical basis for the application of LMWFs as excellent natural antioxidants in various fields such as health food and cosmetics.关键词
低分子质量岩藻聚糖硫酸酯/化学组成/结构特征/抗氧化活性Key words
low molecular mass fucoidans/chemical composition/structure characteristics/antioxidant activity分类
轻工业引用本文复制引用
于凡,郭莹莹,李娜,代心悦,朱文嘉,姚琳,江艳华,王联珠..低分子质量岩藻聚糖硫酸酯的分离纯化、结构表征及体外抗氧化活性[J].食品科学,2025,46(2):50-56,7.基金项目
中国水产科学研究院黄海水产研究所基本科研业务费资助项目(20603022023011) （20603022023011）
现代农业产业技术体系建设专项(CARS-50) （CARS-50）
中国水产科学研究院中央级公益性科研院所基本科研业务费专项(2023TD76) （2023TD76）
