食品科学2025，Vol.46Issue(2)：126-137,12.DOI:10.7506/spkx1002-6630-20240614-096
基于网络药理学探究双孢菇的肝脏保护作用
Exploring the Hepatoprotective Effect of Agaricus bisporus Based on Network Pharmacology
摘要
Abstract
To investigate the hepatoprotective components and mechanism of Agaricus bisporus ethanol extract(ABEE),ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to analyze and identify the components of ABEE.The active components and mechanism were analyzed using network pharmacology and a mouse model of CCl4-induced acute liver injury.UPLC-MS/MS analysis identified 75 compounds,of which 15 were predicted by network pharmacology to be related to liver injury,with 11 different targets.Animal experiments showed that ABEE significantly alleviated CCl4-induced acute liver injury in mice.Compared with the model group,the high-dose group(1 000 mg/kg)showed significant recovery in liver function indicators such as aspartate aminotransferase(AST),alanine aminotransferase(ALT),and alkaline phosphatase(AKP)(P<0.001).The antioxidant capacity in mice was significantly enhanced,as evidenced by a significant increase in superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities(P<0.001)and a significant decrease in malondialdehyde(MDA)content(P<0.01).Detection of the mRNA and protein expression levels of relevant genes was performed using real-time quantitative polymerase chain reaction(PCR)and Western blot techniques,showing that compared with the model group,the expression of the Bcl-2 in the high-dose group significantly increased(P<0.001),while the expression of MYC,NF-κB1,RELA and MMP9 significantly decreased(P<0.01).These results confirmed the predictions,indicating that ABEE might exert its hepatoprotective effect by regulating the expression of Bcl-2,MYC,NF-κB1 and RELA through salicylic acid,palmitic acid,linolenic acid,and chrysin,as well as their associated signaling pathways such as chemical carcinogenesis-receptor activation.This study suggests that ABEE may provide liver protection by inhibiting apoptosis,carcinogenesis,and lipid peroxidation caused by free radicals through multiple components,targets,and pathways.关键词
双孢菇/肝脏保护/网络药理学/超高效液相色谱-串联质谱联用/作用机制Key words
Agaricus bisporus/hepatoprotection/network pharmacology/ultra-high performance liquid chromatography-tandem mass spectrometry/mechanism of action分类
轻工纺织引用本文复制引用
王杰,王金梅,刘慧,刘旭光,郭文静,何红平..基于网络药理学探究双孢菇的肝脏保护作用[J].食品科学,2025,46(2):126-137,12.基金项目
中央引导地方科技发展资金项目(Z20231811055) （Z20231811055）
