食品科学2025，Vol.46Issue(2)：231-239,9.DOI:10.7506/spkx1002-6630-20240718-186
固相萃取-超高效液相色谱-串联质谱法测定蜂蜜中39种植物源性毒素
Simultaneous Determination of 39 Plant Toxins in Honey by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid Phase Extraction
摘要
Abstract
To effectively assess food safety risks associated with potential plant toxin residues present in honey,this study aimed to establish and validate a method for the detection of 39 plant toxins in honey using solid-phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry(SPE-UPLC-MS/MS).The developed method involved sample extraction with 0.1％formic acid,purification using a hydrophilic-lipophilic balanced(HLB)cartridge,detection in the multiple reaction monitoring(MRM)mode,and quantification using the matrix-matched external standard method.The calibration curves exhibited good linearity for all the analytes.Average recoveries ranged from 78.7％to 112.8％with relative standard deviation(RSD)between 1.6％and 15.2％(n=6).This method was characterized by ease of operation,high throughput and high sensitivity,and could be used for the monitoring these toxins in honey.Varying levels of pyrrolizidine alkaloids(PAs)and rhodojaponin Ⅲ were detected in some of the 36 tested batches of honey,confirming the presence of phytotoxin residue risks in honey.关键词
固相萃取/超高效液相色谱-串联质谱/蜂蜜/植物源性毒素Key words
solid-phase extraction/ultra-high performance liquid chromatography-tandem mass spectrometry/honey/plant toxins分类
化学引用本文复制引用
刘川,肖全伟,宋娟,戴琴,陈燕秋,林浩,汪璐瑶,石培育,刘蓉,王义,吴文林,毛锐..固相萃取-超高效液相色谱-串联质谱法测定蜂蜜中39种植物源性毒素[J].食品科学,2025,46(2):231-239,9.基金项目
"十四五"国家重点研发计划重点专项(2022YFF0710405) （2022YFF0710405）
四川省科技计划重点研发项目(2022YFS0511 （）
2023YFS0402) （）
2023年成都市食品安全风险研究项目(2023spfx07) （2023spfx07）
