首页|期刊导航|沈阳农业大学学报|外源6-BA对黄花菜贮藏及生理代谢的影响

外源6-BA对黄花菜贮藏及生理代谢的影响OA北大核心CSTPCD

Effects of Exogenous 6-BA on Preservation and Physiological Metabolism of Hemerocallis citrina Baroni

中文摘要英文摘要

为明确6-BA对黄花菜贮藏和生理代谢的影响,以大同黄花菜为试验材料,喷施不同浓度6-BA(5,10,15 mg·L-1)后进行贮藏,定期取样检测贮藏期内黄花菜的好花率、失重率、叶绿素含量和呼吸强度,筛选出黄花菜贮藏的最佳6-BA浓度,并定期检测该浓度6-BA处理对黄花菜相对电导率、丙二醛、H2O2及抗氧化酶SOD和POD活性的影响,通过相关性分析明确6-BA影响黄花菜生理代谢的机理.结果表明:不同浓度的6-BA处理可降低黄花菜的呼吸强度和失重率,保持较高的好花率,并抑制叶绿素的降解,且10 mg·L-1 6-BA处理对黄花菜的保鲜效果更佳;与对照相比,外施10 mg·L-16-BA可降低黄花菜的相对电导率、丙二醛和H2O2含量,提高SOD和POD酶活性;相关性分析表明黄花菜在衰老过程中,H2O2的积累诱发丙二醛的产生,导致细胞膜通透性增加,即相对电导率增加,而抗氧化酶SOD和POD有效地抑制H2O2含量的增加,减少H2O2对黄花菜的伤害;6-BA处理后抗氧化酶SOD和POD的活性增加,减少H2O2的积累,从而降低了黄花菜在贮藏期间的丙二醛含量和相对电导率.因此,10 mg·L-1 6-BA可延缓采后黄花菜的衰老,达到一定的贮藏保鲜效果,试验结果可为黄花菜贮藏保鲜技术的发展和保鲜机理的研究提供理论依据.

In order to investigate the effect of 6-BA on storage and physiological metabolism of daylily,Datong Daylily was used as experimental material and was sprayed with different concentrations of 6-BA(5,10 and 15 mg·L-1)before storage.Indicator including the good flower rate,weight loss rate,chlorophyll content and respiratory intensity of Datong Daylily were investigated at certain time interval during the storage to select the optimal 6-BA concentration.Moreover,physiological indicators including the relative conductivity,malondialdehyde,H2O2,SOD and POD were investigated.The mechanism of 6-BA affecting the physiological metabolism of daylily was clarified through correlation analysis.The results showed that exogenous 6-BA treatment could reduce the respiratory rate and weight loss rate of daylily,maintain a higher good flower rate,and inhibit the degradation of chlorophyll.Moreover,10 mg·L-1 6-BA treatment has a better preservation effect than other treatments.Compared with the control,application of 10 mg·L-1 6-BA could decrease the relative conductivity,malondialdehyde and H2O2 contents,and increase the activities of SOD and POD enzymes.Correlation analysis showed that the accumulation of H2O2 induced the production of malondialdehyde during the storage of daylily,leading to the increase of cell membrane permeability and relative conductivity.The antioxidant enzymes SOD and POD effectively inhibited the increase of H2O2 content,which reduced the damage of H2O2 to daylily.After 6-BA treatment,the activity of antioxidant enzymes SOD and POD increased,which reduced the accumulation of H2O2,and thus decreased the malondialdehyde content and relative conductivity of daylily during storage.Therefore,exogenous 6-BA treatment could delay the aging of postharvest daylily and achieve certain preservation effect.This study can provide theoretical basis for the development of storage technology and preservation mechanism of daylily.

殷丽丽;张美玲;韩曼丽

山西大同大学农学与生命科学学院,山西 大同 037009北京市农林科学院林业果树研究所,北京 100093山西大同大学农学与生命科学学院,山西 大同 037009

园艺学与植物营养学

黄花菜6-BA贮藏保鲜生理代谢

Hemerocallis citrina Baroni6-BAstoragefresh-keepingphysiological metabolism

《沈阳农业大学学报》 2024 (6)

699-705,7

国家自然科学基金项目(32102340)山西大同大学产学研专项研究项目(2021CXZ8)

10.3969/j.issn.1000-1700.2024.06.006

评论