不同时间和剂量AFB1暴露对小鼠的双向免疫毒性作用OA北大核心CSTPCD

In order to study the time points and dose of aflatoxin B1(AFB1)showing"bidirectional immunotoxicity",the effects of AFB1 exposure at different doses and times on body weight,organ index,autopsy changes and pathological characteristics of mice were investigated by dose gradient test and time gradient test,and the bidirectional immunotoxicity effect of AFB1 exposure on mice was clarified.In the dose gradient test,30 male Kunming mice were randomly divided into 6 groups(5 mice in each group),and daily given 0,10,20,40,80,160 μg·kg-1 of AFB1(by weight),the test lasted for 21 days.In the time gradient test,mice were intraperitoneally injected daily with a fixed dose of AFB1 for 0,7,14,21,28,35 days,respectively.The results showed that mice exposed to higher concentrations(80,160 μg·kg-1)and a longer period(21 days)of AFB1 had significantly lower weight gain(p<0.01),and the expression levels of TNF-α,spleen index and thymus index increased first and decreased then than those exposed to the control group(0 μg·kg-1),while IL-10 showed a significant increase in higher concentrations(80,160 μg·kg-1)and longer period(21 days)of AFB1 exposure,and the degree of damage to the spleen and thymus also showed similar results.In conclusion,low concentrations or short periods of AFB1 exposure may induce inflammation,while high concentrations or prolonged AFB1 exposure may lead to immunosuppression,which confirms that different times and doses of AFB1 exposure can induce bidirectional immunotoxic effects,and the results of this study will provide a scientific basis for the prevention and control of AFB1 pollution and the in-depth study of the pathogenic mechanism.