茶叶科学2024，Vol.44Issue(6)：869-886,18.
茶树果胶甲酯酶及其抑制子家族基因的鉴定及CsPME55参与氟胁迫响应的功能分析
Identification of Pectin Methylesterase and Its Inhibitory Subfamily Genes,and Functional Analysis of CsPME55 in Response to Fluoride Stress in Camellia sinensis
摘要
Abstract
Tea plant(Camellia sinensis)has fluoride(F)enrichment characteristics,and F is mainly enriched in the cell wall component pectin.Pectin methylsterase(PME)and its inhibitor PMEI can catalyze the modification of pectin,thereby affecting cell wall characteristics and participating in the regulation of processes like plant growth and development,stress response and so on.In this study,85 CsPMEs and 56 CsPMEIs were identified from the C.sinensis'Shuchazao'genome,which were divided into 4 and 5 subgroups,respectively.Distinct subgroups may exhibit functional distinction due to varied gene architectures,conserved motifs and expression patterns.Quantitative real-time PCR(qRT-PCR)analysis reveals that the expression levels of CsPME3a,CsPME55,CsPMEI1 and CsPMEI3 were significantly induced in the mature leaves of'Fuding Dabaicha'under F treatment.Moreover,overexpression of CsPME55 alleviated Arabidopsis root growth inhibition induced by F stress,suggesting its potential role in F stress regulation in tea plants.These findings could pave the way for further research on the functional involvement of PME and PMEI gene families in F response.关键词
茶树/氟/果胶甲酯酶/果胶甲酯酶抑制子/基因表达/功能分析Key words
Camellia sinensis/fluoride/pectin methylesterase/pectin methylesterase inhibitor/gene expression/functional analysis分类
农业科技引用本文复制引用
徐文鸾,温晓菊,贾雨轩,倪德江,王明乐,陈玉琼..茶树果胶甲酯酶及其抑制子家族基因的鉴定及CsPME55参与氟胁迫响应的功能分析[J].茶叶科学,2024,44(6):869-886,18.基金项目
国家自然科学基金项目(31470691) （31470691）
