茶叶科学2024，Vol.44Issue(6)：1005-1013,9.
陈年六堡茶对Aβ25-35诱导的PC12细胞损伤的神经保护研究
Neuroprotective Mechanisms of Aged Liupao Tea against Aβ25-35-induced PC12 Cell Damage
摘要
Abstract
In this study,an Aβ25-35-induced PC12 cell damage model was established to investigate the neuroprotective effects and underlying mechanisms of aged Liupao tea(ALPT),with green tea(GT)as a reference.The results show that Aβ25-35 significantly reduced PC12 cell viability,induced mitochondrial dysfunction,and promoted the formation of toxic aggregates and related pathways.ALPT markedly improved cell survival,increased mitochondrial membrane potential,and significantly inhibited the accumulation of toxic aggregates and the formation of related pathways.Furthermore,transcriptome analysis reveals that the overall gene expression pattern in the ALPT treatment group was the opposite to that in the Aβ25-35 group,with upregulated genes involved in mitophagy,glycolysis and glycerophospholipid metabolism,and downregulated genes associated with cell cycle regulation,ribosomal function,ubiquitin-mediated proteolysis and cellular senescence.Overall,both GT and ALPT exhibited significant protective effects against Aβ25-35-induced PC12 cell damage,though transcriptomic differences suggest that ALPT may have superior bioavailability due to its active components.This study provided experimental evidence for the potential application of ALPT in the prevention and treatment of neurodegenerative diseases.关键词
陈年六堡茶/β-淀粉样蛋白25-35/PC12细胞/神经保护/线粒体功能Key words
aged Liupao tea/Aβ25-35/PC12 cells/neuroprotective/mitochondrial function分类
农业科技引用本文复制引用
聂晴,庞月兰,吴焕,丁树洽,钟可渝,刘仲华,蔡淑娴..陈年六堡茶对Aβ25-35诱导的PC12细胞损伤的神经保护研究[J].茶叶科学,2024,44(6):1005-1013,9.基金项目
广西创新驱动发展专项资金项目(AA20302018)、广西茶叶试验站(TS202106)、国家重点研发计划(2018YFC1604405)、国家自然科学基金项目(31471590、31100501) （AA20302018）
