食品工业科技2025，Vol.46Issue(2)：35-44,10.DOI:10.13386/j.issn1002-0306.2023110113
不同植物多酚-β-乳球蛋白复合物的抗原性、水溶性与消化性分析
Antigenicity,Solubility and Digestibility of β-Lactoglobulin Combined with Different Plant Polyphenols
摘要
Abstract
Plant polyphenols were exploited in this research to reduce the antigenicity of β-lactoglobulin based on their mutual interaction(physical combination),in an effort to evaluate its potential on the application of new technics to fabricate hypoallergenic lactoproteins.In this study,non-covalent,alkaline,and enzymatic methods,were adopted to construct the complex or conjugated compounds of β-lactoglobulin combined by plant phenolic extracts from tea residue,mulberry pomace,longan pericarp,longan seed,grape seed and grape pomace.By means of the determination of allergenicity in vitro(through enzyme linked immunosorbent assay and immunoblotting),the six kind of plant polyphenols extracts could reduce the antigenicity(specific IgG binding capacity)of β-lactoglobulin to different extents(under alkaline and enzymatic treatment),in which longan pericarp and seed polyphenols exhibited the best(the mean of antigenic inhibition rate:76.8％ ,77.2％ ,respectively).Meanwhile,liquid chromatography coupled with tandem mass spectrometry indicated that gallic acid unit-based tannins(or acids),such as corilagin and ellagic acid,were dominant polyphenols in longan pericarp/seed extracts.In addition,gel electrophoresis revealed that six extracts induced aggregation between proteins,forming dimers or polymers.The solubility of the complexes or adducts constructed via enzymatic catalytic process was lower than the corresponding compounds built by non-covalent or alkaline methods(except for tea residue polyphenols group).Nonetheless,most of the extracts did not apparently affect the in vitro global digestibility of the protein,apart from the grape pomace treatment under alkaline condition.Taken the results of desensitization efficiency and functionality and digestibility into account,this study could preliminarily conclude that phenols stemmed from longan pericarp and seed might be the suitable materials in suppressing the allergenicity of β-lactoglobulin.关键词
植物多酚/β-乳球蛋白/脱敏/水溶性/消化性/多酚组成Key words
plant polyphenols/β-lactoglobulin/desensitization/solubility/digestibility/polyphenol composition分类
轻工纺织引用本文复制引用
农丽艳,刘学铭,程镜蓉,邹金浩,曹文红,唐道邦,杨怀谷..不同植物多酚-β-乳球蛋白复合物的抗原性、水溶性与消化性分析[J].食品工业科技,2025,46(2):35-44,10.基金项目
广州市基础研究计划基础与应用基础研究项目(202201010671) （202201010671）
广东省农业科学院科技人才引进专项资金项目(R2021YJ-YB3008) （R2021YJ-YB3008）
广东省重点领域研发计划项目(2022B0202030002) （2022B0202030002）
广东省农业科研类及科技推广示范类项目(44000220000000017411) （44000220000000017411）
广东省现代农业产业技术体系创新团队(2019KJ114). （2019KJ114）
