食品工业科技2025，Vol.46Issue(2)：175-183,9.DOI:10.13386/j.issn1002-0306.2024030064
基于三代测序技术高温和中高温大曲真菌多样性解析及酵母菌的分离鉴定
Analysis of Fungal Diversity and Isolation and Identification of Yeast in High-temperature and Medium-high-temperature Daqu Using Three Generation Sequencing Technology
摘要
Abstract
To elucidate the variations in fungal diversity and cultivable yeast in different types of Daqu,third-generation sequencing technology was employed to analyze the fungal communities in high-temperature and medium-high-temperature Daqu.Additionally,traditional microbial pure culture methods and molecular biology techniques were utilized to investigate the yeast resources within Daqu.Alpha diversity analysis revealed significant differences(P＜0.05)in the richness and diversity of fungal communities between the two types of Daqu.The sequencing data indicated that Thermomyces lanuginosus predominated in high-temperature Daqu,with an average relative content of 73.81％ ,while medium-high-temperature Daqu was primarily composed of Aspergillus proliferans,representing an average relative content of 52.58％ .Beta diversity analysis demonstrated a notable distinction in the fungal community structure between high-temperature and medium-high-temperature Daqu(P＜0.01).Moreover,the LEfSe analysis identified Thermomyces and Aspergillaceae as potential biomarkers for high-temperature and medium-high-temperature Daqu,respectively.Pure culture results revealed 18 yeast strains isolated from 10 Daqu samples,with Saccharomycopsis fibuligera identified as the dominant yeast strain in both high-temperature and medium-high-temperature Daqu.These findings underscore pronounced differences in fungal diversity between high-temperature and medium-high-temperature Daqu.关键词
高温大曲/中高温大曲/三代测序技术/真菌类群Key words
high-temperature Daqu/medium-high-temperature Daqu/third generation sequencing technology/fungal taxa分类
轻工业引用本文复制引用
崔梦君,王玉荣,侯强川,张海波,田龙新,叶明波,郭壮..基于三代测序技术高温和中高温大曲真菌多样性解析及酵母菌的分离鉴定[J].食品工业科技,2025,46(2):175-183,9.基金项目
湖北省教育厅优秀中青年科技创新团队(T2023022) （T2023022）
湖北文理学院教师科研能力培育基金"科技创新团队"(2020kypytd009). （2020kypytd009）
