食品工业科技2025，Vol.46Issue(2)：184-191,8.DOI:10.13386/j.issn1002-0306.2023120080
微波辅助萃取油松松针精油的工艺优化及抗氧化活性研究
Process Optimization and Antioxidant Activity of Pine Needle Essential Oil Extracted by Microwave-assisted Extraction
摘要
Abstract
Objective:To optimize the best process for the extraction of pine needle essential oil from Pinus tabuliformis,and to conduct its compositional analysis and antioxidant capacity.Methods:Response surface methodology was used to optimize the process of extracting the pine needle essential oil from Pinus tabuliformis by microwave-assisted extraction(MAE),the components of essential oil were identified by gas chromatography-mass spectrometry(GC-MS)and the antioxidant activity was verified by DPPH and ABTS+free radicals scavenging ability as indicators.Results:The optimal extraction processes were as follows:extraction time 55 min,microwave power 550 W,liquid-to-solid ratio 10∶1 mL/g,the predicted yield was 0.245％ ,and the validation result of the optimized process was 0.238％ .A total of 104 candidate components were obtained from the essential oil,with major components including thunbergol(3.938％ ),and verticillol(3.597％ ),and so on.In the antioxidant experiment,L-ascorbic acid was used as a control to determine the DPPH and ABTS+free radicals scavenging ability,and the half maximal inhibitory concentration(IC50)was 101.74 and 12.39 mg/mL,respectively.Meanwhile,at the concentration of essential oil of 128.00 mg/mL,the DPPH free radical scavenging rate could reach 64.92％ and the ABTS+free radical scavenging rate could reach 84.11％ .Conclusion:By optimizing the microwave-assisted extraction process of pine needle essential oil from Pinus tabuliformis,and measuring the main volatile components of pine needle essential oil obtained by this method,its antioxidant activity was confirmed,which would provide theoretical support for the development of green and efficient extraction methods of pine needle essential oil and further utilization of pine needle essential oil resources.关键词
油松/松针精油/微波辅助萃取/响应面分析/抗氧化Key words
Pinus tabuliformis/pine needle essential oil/microwave-assisted extraction/response surface methodology/antioxidant分类
林学引用本文复制引用
吕素媛,商冰清,孙璐颜,刘国龙,赵发,梁秀清,吴秋,耿越..微波辅助萃取油松松针精油的工艺优化及抗氧化活性研究[J].食品工业科技,2025,46(2):184-191,8.基金项目
山东省自然科学基金青年项目(ZR2023QC105). （ZR2023QC105）
