食品工业科技2025，Vol.46Issue(2)：208-217,10.DOI:10.13386/j.issn1002-0306.2024020046
杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析
Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus
摘要
Abstract
In this study,an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures.By conducting single-factor experiments,Plackett-Burman design,and response surface design,the impact of 12 factors on co-culture intracellular polysaccharide(CC-IPS)extraction content,including extraction material-liquid ratios,extraction time,extraction temperature,and extraction solution pH were investigated.The antioxidant capacity of CC-IPS in vitro was evaluated by the DPPH and ABTS+radical scavenging rates.Results showed that,the optimal extraction process parameters for the first,second,and third extractions,respectively,included material-to-liquid ratios of 1∶17,1∶40,and 1∶30(g/mL),extraction times of 31,30,and 30 min,extraction temperatures of 44,40,and 50 ℃,and extraction solution pH levels of 3,8,and 10.Under these optimal conditions,the CC-IPS extraction content was 86.4921±1.1924 mg/g,closed to the predicted value of 85.3981 mg/g.Additionally,the IC50 values of CC-IPS in the DPPH and ABTS+free radical scavenging assays were determined to be 0.2425 and 0.1376 mg/mL,respectively.This study offers a theoretical foundation for the efficient utilization of intracellular polysaccharides in liquid fungal co-cultures.关键词
杨树桑黄/紫孢侧耳/半仿生提取法/共培养/多糖/抗氧化活性Key words
Sanghuangporus vaninii/Pleurotus sapidus/semi-bionic extraction/co-culture/polysaccharide/antioxidant activity分类
轻工业引用本文复制引用
陆源添,刘迪..杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析[J].食品工业科技,2025,46(2):208-217,10.基金项目
国家重点研发项目(2017YFD0300104) （2017YFD0300104）
中韩国际农业技术合作项目(延大横向[2019]第19号). （延大横向[2019]第19号）
