食品工业科技2025，Vol.46Issue(2)：324-333,10.DOI:10.13386/j.issn1002-0306.2024010212
黑豆-乳清双蛋白膳食对脂多糖诱导肠屏障损伤大鼠的保护作用
Protective Effect of Black Bean-Whey Double Protein Diet on Intestinal Barrier Injury Induced by Lipopolysaccharide in Rats
摘要
Abstract
To explore the protective effects of a black bean-whey double protein(B-W double protein,B-W DP)diet on intestinal barrier injury induced by lipopolysaccharides(LPS)in rats.Sprague-Dawley rats were fed low,medium,and high doses of B-W DP for 28 d,and then,LPS was used to induce intestinal barrier injury.The intestinal morphology of the rats was observed using hematoxylin and eosin staining.The expression of tight junction proteins was quantified using immunohistochemistry,and the TLR4 and MyD88 expression levels were determined using real-time fluorescence quantitative polymerase chain reaction.Results showed that compared with low-and high-dose B-W DP,intermediate-dose B-W DP significantly(P＜0.01)protected against intestinal barrier injury caused by LPS,increased villus length by 26.69％ ,reduced crypt depth by 22.61％ ,and increased their ratio by 46.78％ .Three doses of B-W DP regulated tight junction proteins in different manners,and the intermediate dose exhibited the most significant effect(P＜0.01),increasing ZO-1,Claudin-1,and Occludin expression levels by 14.32％ ,31.80％ ,and 16.67％ ,respectively.Three doses of B-W DP significantly inhibited the inflammatory pathway(P＜0.01),and the intermediate dose exhibited the most significant effect,reducing TLR4 and MyD88 protein levels by 37.25％ and 33.04％ ,respectively.In summary,a moderate dose of B-W DP can improve intestinal morphology,intestinal digestion,and absorption capacity,increase the expression of tight junction proteins,maintain the integrity of epithelial cells,reduce intestinal permeability,downregulate TLR4 and MyD88 protein expression,and alleviate inflammatory reactions.All of which exhibit effective protective effects on LPS-induced intestinal barrier injury.关键词
黑豆-乳清双蛋白/脂多糖/肠屏障损伤/肠道形态/紧密连接蛋白Key words
black bean-whey double protein/lipopolysaccharide/intestinal barrier injury/intestinal morphology/tight junction protein分类
轻工纺织引用本文复制引用
孟维洪,李志明,张家瑜,舒欣,梁得福,庄柯瑾,张东杰..黑豆-乳清双蛋白膳食对脂多糖诱导肠屏障损伤大鼠的保护作用[J].食品工业科技,2025,46(2):324-333,10.基金项目
国家重点研发计划项目(2023YFD1202705-6) （2023YFD1202705-6）
黑龙江省杂粮生产与加工优势特色学科项目(2022-78) （2022-78）
黑龙江省自然科学基金资助项目(LH2024C087) （LH2024C087）
黑龙江省"优秀青年教师基础研究支持计划"资助项目(YQJH2024168). （YQJH2024168）
