基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用OA北大核心

Purpose:To explore the laxative effect of Lacticaseibacillus rhamnosus Glory LG12 from the perspective of the stem cell factor(SCF)/C-kit signaling pathway.Methods:Male ICR mice were randomly divided into five groups:control,model,low-(1.5×106 CFU/mouse),medium-(1.5×107 CFU/mouse),and high-(1.5×108 CFU/mouse)dose L.rhamnosus Glory LG12.After 15 continuous days of gavage,all mice were further gavaged with loperamide hydrochloride suspension(4 mg/kg)to establish a mouse model of constipation.Body mass,the time taken until the first black stool excretion,the number and mass of black stool within 5 hours,small intestinal transit rate,serum levels of gastrointestinal regulatory peptides and inflammatory cytokines,colonic SCF and C-kit transcription levels were measured,and colonic pathology was examined.Results:Compared with the model group,medium-and high-dose L.rhamnosus Glory LG12 significantly reduced the time taken until the first black stool excretion(P<0.001),increased the number and mass of black stools within 5 h(P<0.01 and P<0.001,respectively),and improved the ink propulsion rate in the small intestine(P<0.001).In addition,both treatments elevated the serum levels of motilin(MTL),gastrin(Gas),substance P(SP),and interleukin 10(IL-10),reduced the levels of endothelin-1(ET-1),somatostatin(SS),vasoactive intestinal peptide(VIP),and IL-6,up-regulated the relative mRNA expression of SCF and C-kit in colonic tissue was(P<0.01,P<0.001),and restored damaged colonic barrier.Conclusion:L.rhamnosus Glory LG12 promotes gastrointestinal motility by affecting the release of gastrointestinal regulatory peptides and inflammatory cytokines through the modulation of the SCF/C-kit signaling pathway,thereby alleviating constipation symptoms.