食品科学2025，Vol.46Issue(3)：20-26,7.DOI:10.7506/spkx1002-6630-20240710-109
基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用
Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12:from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway
摘要
Abstract
Purpose:To explore the laxative effect of Lacticaseibacillus rhamnosus Glory LG12 from the perspective of the stem cell factor(SCF)/C-kit signaling pathway.Methods:Male ICR mice were randomly divided into five groups:control,model,low-(1.5×106 CFU/mouse),medium-(1.5×107 CFU/mouse),and high-(1.5×108 CFU/mouse)dose L.rhamnosus Glory LG12.After 15 continuous days of gavage,all mice were further gavaged with loperamide hydrochloride suspension(4 mg/kg)to establish a mouse model of constipation.Body mass,the time taken until the first black stool excretion,the number and mass of black stool within 5 hours,small intestinal transit rate,serum levels of gastrointestinal regulatory peptides and inflammatory cytokines,colonic SCF and C-kit transcription levels were measured,and colonic pathology was examined.Results:Compared with the model group,medium-and high-dose L.rhamnosus Glory LG12 significantly reduced the time taken until the first black stool excretion(P<0.001),increased the number and mass of black stools within 5 h(P<0.01 and P<0.001,respectively),and improved the ink propulsion rate in the small intestine(P<0.001).In addition,both treatments elevated the serum levels of motilin(MTL),gastrin(Gas),substance P(SP),and interleukin 10(IL-10),reduced the levels of endothelin-1(ET-1),somatostatin(SS),vasoactive intestinal peptide(VIP),and IL-6,up-regulated the relative mRNA expression of SCF and C-kit in colonic tissue was(P<0.01,P<0.001),and restored damaged colonic barrier.Conclusion:L.rhamnosus Glory LG12 promotes gastrointestinal motility by affecting the release of gastrointestinal regulatory peptides and inflammatory cytokines through the modulation of the SCF/C-kit signaling pathway,thereby alleviating constipation symptoms.关键词
鼠李糖乳酪杆菌Glory LG12/便秘/润肠通便效果/炎性细胞因子/胃肠调节肽/干细胞因子/C-kit信号通路Key words
Lacticaseibacillus rhamnosus Glory LG12/constipation/laxative effect/inflammatory cytokines/gastrointestinal regulatory peptides/stem cell factor/C-kit signaling pathway分类
轻工业引用本文复制引用
项芳琴,李玉星,李柏良..基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用[J].食品科学,2025,46(3):20-26,7.基金项目
中国博士后科学基金特别资助项目(2024T140106) （2024T140106）
国家自然科学基金青年科学基金项目(32101919) （32101919）
